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Literature summary extracted from

  • Doi, Y.; Lee, B.R.; Ikeguchi, M.; Ohoba, Y.; Ikoma, T.; Tero-Kubota, S.; Yamauchi, S.; Takahashi, K.; Ichishima, E.
    Substrate specificities of deuterolysin from Aspergillus oryzae and electron paramagnetic resonance measurement of cobalt-substituted deuterolysin (2003), Biosci. Biotechnol. Biochem., 67, 264-270.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.39 0.0119
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0237
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.0264
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.0275
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0473
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.0522
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0638
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.07
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.146
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.1573
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.39 Zn2+ zinc-protease, contains one mole of zinc per mole of enzyme Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.39 19000
-
-
 Aspergillus oryzae
3.4.24.39 19020
-
 amino acid sequence Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.39 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.39
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.39 calf thymus histone 4 + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 additional information almost no hydrolysis toward mono or certain dibasic peptide 4-methylcoumarin-7-amides like N-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ile-Glu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide, Z-Phe-Arg-4-methylcoumarin-7-amide, Arg-4-methylcoumarin-7-amide, Lys-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Arg-Arg-4-methylcoumarin-7-amide, and N-butoxycarbonyl-Gln-Lys-Lys-4-methylcoumarin-7-amide Aspergillus oryzae ?
-
 ?
3.4.24.39 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Z-Arg-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.39 30 100 extremely stable at 100°C, but unstable near 75°C because of self-digestion Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.39 0.00065
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.00082
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0009
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.0017
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0022
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.0035
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.0045
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.009
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
3.4.24.39 0.017
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
3.4.24.39 0.022
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.39 9
-
-
 Aspergillus oryzae