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Literature summary extracted from

  • Sakai, T.; Daikai, T.; Monma, H.; Maeda, H.
    Isolation from Nocardioides sp. strain CT16, purification, and characterization of a deoxycytidine deaminase extremely thermostable in the presence of DL-dithiothreitol (2002), Biosci. Biotechnol. Biochem., 66, 1646-1651.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.055
-
 dCMP pH 7.0, 37°C Nocardioides sp.
3.5.4.5 0.13
-
 5-methyl-dCMP pH 7.0, 37°C Nocardioides sp.
3.5.4.5 0.14
-
 CMP pH 7.0, 37°C Nocardioides sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 18000
-
 2 * 18000, SDS-PAGE Nocardioides sp.
3.5.4.5 52000
-
 gel filtration Nocardioides sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Nocardioides sp.
-
-
-
3.5.4.5 Nocardioides sp. CT16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Nocardioides sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 225
-
 pH 7.0, 37°C Nocardioides sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 5-methyl-dCMP + H2O
-
 Nocardioides sp. 5-methyl-dCyd
-
 ?
3.5.4.5 5-methyl-dCMP + H2O
-
 Nocardioides sp. CT16 5-methyl-dCyd
-
 ?
3.5.4.5 CMP + H2O
-
 Nocardioides sp. ?
-
 ?
3.5.4.5 CMP + H2O
-
 Nocardioides sp. CT16 ?
-
 ?
3.5.4.5 CTP + H2O
-
 Nocardioides sp. UTP + NH3
-
 ?
3.5.4.5 CTP + H2O
-
 Nocardioides sp. CT16 UTP + NH3
-
 ?
3.5.4.5 dCMP + H2O
-
 Nocardioides sp. ?
-
 ?
3.5.4.5 dCMP + H2O
-
 Nocardioides sp. CT16 ?
-
 ?
3.5.4.5 dCTP + H2O
-
 Nocardioides sp. dUTP + NH3
-
 ?
3.5.4.5 dCTP + H2O
-
 Nocardioides sp. CT16 dUTP + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 18000, SDS-PAGE Nocardioides sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 99
-
 maximum of activity Nocardioides sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.5 50
-
 15 min, absence of dithiothreitol, 44% residual activity Nocardioides sp.
3.5.4.5 100
-
 1 h, 1 mM dithiothreitol, 48% residual activity Nocardioides sp.
3.5.4.5 100
-
 1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity Nocardioides sp.
3.5.4.5 100
-
 15 min, 1 mM dithiothreitol, 80% residual activity Nocardioides sp.
3.5.4.5 100
-
 2 h, 50 mM dithiothreitol, 56% residual activity Nocardioides sp.