EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.11 | expression in Escherichia coli | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.4.1.11 | Y114F | 0.1% of wild-type kcat for L-methionine | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.11 | 0.27 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 0.4 | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 0.48 | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 0.9 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 2.22 | - |
O-acetyl-L-homoserine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 7.22 | - |
L-Vinylglycine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 8.22 | - |
L-methionine sulfone | 37°C, pH 8.0 | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.11 | L-methionine + H2O | Pseudomonas putida | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.11 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.11 | recombinant L-methionine gamma-lyase | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.11 | L-ethionine + H2O | - |
Pseudomonas putida | ethanethiol + NH3 + 2-oxobutanoate | - |
? | |
4.4.1.11 | L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
4.4.1.11 | L-methionine sulfone | - |
Pseudomonas putida | ? | - |
? | |
4.4.1.11 | L-vinylglycine | - |
Pseudomonas putida | ? | - |
? | |
4.4.1.11 | O-acetyl-L-homoserine | - |
Pseudomonas putida | ? | - |
? | |
4.4.1.11 | S-ethyl-L-cysteine | - |
Pseudomonas putida | ? | - |
? | |
4.4.1.11 | S-methyl-L-cysteine | - |
Pseudomonas putida | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.11 | additional information | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | additional information | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 0.017 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 0.17 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 5.53 | - |
S-methyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 5.79 | - |
S-ethyl-L-cysteine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 33.4 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 40.4 | - |
L-methionine sulfone | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 44.4 | - |
L-Vinylglycine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 48.6 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
4.4.1.11 | 78 | - |
O-acetyl-L-homoserine | 37°C, pH 8.0 | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.11 | pyridoxal 5'-phosphate | - |
Pseudomonas putida |