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Literature summary extracted from

  • Seow, T.K.; Inagaki, K.; Tamura, T.; Soda, K.; Tanaka, H.
    Alanine racemase from an acidophile, Acidiphilium organovorum: purification and characterization (1998), Biosci. Biotechnol. Biochem., 62, 242-247.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 D-penicillamine 1 mM, 79% inhibition Acidiphilium organovorum
5.1.1.1 hydroxylamine 1 mM, 68% inhibition Acidiphilium organovorum
5.1.1.1 L-Penicillamine 1 mM, 28% inhibition Acidiphilium organovorum
5.1.1.1 NaBH4 0.5 M, loss of activity Acidiphilium organovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 3.8
-
D-Ala pH 9.0 Acidiphilium organovorum
5.1.1.1 7.9
-
L-Ala pH 9.0 Acidiphilium organovorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.1 cytoplasm
-
Acidiphilium organovorum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 33000
-
2 * 33000, SDS-PAGE Acidiphilium organovorum
5.1.1.1 65000
-
gel filtration Acidiphilium organovorum

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Acidiphilium organovorum
-
13H
-
5.1.1.1 Acidiphilium organovorum 13H
-
13H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
Acidiphilium organovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 191
-
-
Acidiphilium organovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala highly specific for Ala Acidiphilium organovorum D-Ala
-
r
5.1.1.1 L-Ala highly specific for Ala Acidiphilium organovorum 13H D-Ala
-
r

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 33000, SDS-PAGE Acidiphilium organovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 50 60
-
Acidiphilium organovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.1 65
-
30 min, no loss of activity Acidiphilium organovorum
5.1.1.1 70
-
30 min, enzyme loses most of its activity Acidiphilium organovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 9
-
-
Acidiphilium organovorum

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate 1 mol of enzyme contains 2 mol of cofactor Acidiphilium organovorum