EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | F- | 200 mM, activation to 160.7% of control | Aspergillus niger | |
3.1.3.26 | F- | 200 mM, activation to 160.7% of control | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Ca2+ | 200 mM, 32% inhibition | Aspergillus niger | |
3.1.3.26 | Ca2+ | 200 mM, 32% inhibition | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.1 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger | |
3.1.3.26 | 0.1 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.8 | extracellular | - |
Aspergillus niger | - |
- |
3.1.3.26 | extracellular | - |
Aspergillus niger | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Cd2+ | 200 mM, activation to 156% of control | Aspergillus niger | |
3.1.3.8 | Cu2+ | 200 mM, activation to 160% of control | Aspergillus niger | |
3.1.3.8 | Hg2+ | 200 mM, activation to 158% of control | Aspergillus niger | |
3.1.3.8 | Mg2+ | 200 mM, activation to 154% of control | Aspergillus niger | |
3.1.3.8 | Mn2+ | 200 mM, activation to 147.8% of control | Aspergillus niger | |
3.1.3.8 | additional information | no effect is observed after addition of 200 mM Fe2+ or Fe3+ | Aspergillus niger | |
3.1.3.8 | Zn2+ | 200 mM, activation to 142.7% of control | Aspergillus niger | |
3.1.3.26 | Cd2+ | 200 mM, activation to 156% of control | Aspergillus niger | |
3.1.3.26 | Cu2+ | 200 mM, activation to 160% of control | Aspergillus niger | |
3.1.3.26 | Hg2+ | 200 mM, activation to 158% of control | Aspergillus niger | |
3.1.3.26 | Mg2+ | 200 mM, activation to 154% of control | Aspergillus niger | |
3.1.3.26 | Mn2+ | 200 mM, activation to 147.8% of control | Aspergillus niger | |
3.1.3.26 | additional information | no effect is observed after addition of 200 mM Fe2+ or Fe3+ | Aspergillus niger | |
3.1.3.26 | Zn2+ | 200 mM, activation to 142.7% of control | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 84000 | - |
1 * 84000, SDS-PAGE | Aspergillus niger |
3.1.3.8 | 87000 | - |
gel filtration | Aspergillus niger |
3.1.3.26 | 84000 | - |
1 * 84000, SDS-PAGE | Aspergillus niger |
3.1.3.26 | 87000 | - |
gel filtration | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Aspergillus niger | - |
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
3.1.3.26 | Aspergillus niger | - |
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.8 | glycoprotein | 31% carbohydrate | Aspergillus niger |
3.1.3.26 | glycoprotein | 31% carbohydrate | Aspergillus niger |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | - |
Aspergillus niger |
3.1.3.26 | - |
Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5.276 | - |
- |
Aspergillus niger |
3.1.3.26 | 5.276 | - |
- |
Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | 1-naphthyl phosphate + H2O | 74% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 1-naphthol + phosphate | - |
? | |
3.1.3.8 | 2-naphthyl phosphate + H2O | 63.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 2-naphthol + phosphate | - |
? | |
3.1.3.8 | 4-nitrophenyl phosphate + H2O | 39.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | ADP + H2O | 25.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
3.1.3.8 | AMP + phosphate | 43.9% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | adenosine + phosphate | - |
? | |
3.1.3.8 | ATP + H2O | 38.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
3.1.3.8 | D-glucose 1-phosphate + H2O | 28.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | D-glucose + phosphate | - |
? | |
3.1.3.8 | glycerol 2-phosphate + H2O | 50.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | glycerol + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? | |
3.1.3.8 | phenyl phosphate + H2O | 84% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | phenol + phosphate | - |
? | |
3.1.3.26 | 1-naphthyl phosphate + H2O | 74% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 1-naphthol + phosphate | - |
? | |
3.1.3.26 | 2-naphthyl phosphate + H2O | 63.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 2-naphthol + phosphate | - |
? | |
3.1.3.26 | 4-nitrophenyl phosphate + H2O | 39.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
3.1.3.26 | ADP + H2O | 25.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
3.1.3.26 | AMP + phosphate | 43.9% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | adenosine + phosphate | - |
? | |
3.1.3.26 | ATP + H2O | 38.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
3.1.3.26 | D-glucose 1-phosphate + H2O | 28.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | D-glucose + phosphate | - |
? | |
3.1.3.26 | glycerol 2-phosphate + H2O | 50.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | glycerol + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? | |
3.1.3.26 | phenyl phosphate + H2O | 84% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | phenol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | monomer | 1 * 84000, SDS-PAGE | Aspergillus niger |
3.1.3.26 | monomer | 1 * 84000, SDS-PAGE | Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 65 | - |
- |
Aspergillus niger |
3.1.3.26 | 65 | - |
- |
Aspergillus niger |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 45 | 75 | 45°C: about 45% of maximal activity, 75°C: about 50% of maximal activity | Aspergillus niger |
3.1.3.26 | 45 | 75 | 45°C: about 45% of maximal activity, 75°C: about 50% of maximal activity | Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 80 | - |
3 min, 78% loss of activity | Aspergillus niger |
3.1.3.26 | 80 | - |
3 min, 78% loss of activity | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5 | - |
and a second lower pH-optimum at pH 11.5 | Aspergillus niger |
3.1.3.8 | 11.5 | - |
and a second higher pH-optimum at pH 11.5 | Aspergillus niger |
3.1.3.26 | 5 | - |
and a second lower pH-optimum at pH 11.5 | Aspergillus niger |
3.1.3.26 | 11.5 | - |
and a second higher pH-optimum at pH 5.0 | Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 2 | 12 | pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity | Aspergillus niger |
3.1.3.26 | 2 | 12 | pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity | Aspergillus niger |