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Literature summary extracted from

  • Kelley-Loughnane, N.; Biolsi, S.A.; Gibson, K.M.; Lu, G.; Hehir, M.J.; Phelan, P.; Kantrowitz, E.R.
    Purification, kinetic studies, and homology model of Escherichia coli fructose-1,6-bisphosphatase (2002), Biochim. Biophys. Acta, 1594, 6-16.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP
-
 Escherichia coli
3.1.3.11 AMP
-
 Sus scrofa
3.1.3.11 D-fructose 2,6-bisphosphate
-
 Escherichia coli
3.1.3.11 D-fructose 2,6-bisphosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.0014
-
 D-fructose 1,6-bisphosphate pH 7.5, 30°C Sus scrofa
3.1.3.11 0.0154
-
 D-fructose 1,6-bisphosphate pH 7.5, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required, KM of 0.34 mM Sus scrofa
3.1.3.11 Mg2+ required, KM of 0.62 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 38600
-
 4 * 38600, SDS-PAGE Escherichia coli
3.1.3.11 136000
-
 gel filtration Sus scrofa
3.1.3.11 150000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Escherichia coli enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Escherichia coli
-
-
-
3.1.3.11 Sus scrofa P00636
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 50% Sus scrofa
3.1.3.11 purity of 98% Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 24.2
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Escherichia coli D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Escherichia coli D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 tetramer
-
 Sus scrofa
3.1.3.11 tetramer 4 * 38600, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 14.6
-
 D-fructose 1,6-bisphosphate pH 7.5, 30°C Escherichia coli
3.1.3.11 21
-
 D-fructose 1,6-bisphosphate pH 7.5, 30°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 7.8
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.11 0.000065
-
 D-fructose 2,6-bisphosphate pH 7.5, 30°C Sus scrofa
3.1.3.11 0.0003
-
 D-fructose 2,6-bisphosphate pH 7.5, 30°C Escherichia coli