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Literature summary extracted from

  • Holden, M.J.; Mayhew, M.P.; Gallagher, D.T.; Vilker, V.L.
    Chorismate lyase: kinetics and engineering for stability (2002), Biochim. Biophys. Acta, 1594, 160-167.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.40 C14S/C81S mutation causes greatly improved solution behavior and minor effect on enzyme activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.40 3-carboxymethylaminomethyl-4-hydroxybenzoate
-
 Escherichia coli
4.1.3.40 4-hydroxybenzaldehyde
-
 Escherichia coli
4.1.3.40 4-hydroxybenzoate product inhibition Escherichia coli
4.1.3.40 vanillate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.40 0.028
-
 chorismate pH 7.5, wild-type enzyme, initial rate analysis Escherichia coli
4.1.3.40 0.029
-
 chorismate pH 7.5, wild-type enzyme, progress curve analysis Escherichia coli
4.1.3.40 0.037
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, initial rate analysis Escherichia coli
4.1.3.40 0.039
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, progress curve analysis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.40 chorismate Escherichia coli ubiquinone biosynthetic pathway 4-hydroxybenzoate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.40 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.40 wild-type and mutant enzyme C14S/C81S Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.40 chorismate ubiquinone biosynthetic pathway Escherichia coli 4-hydroxybenzoate + pyruvate
-
 ?
4.1.3.40 chorismate +
-
 Escherichia coli 4-hydroxybenzoate + pyruvate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.40 1.3
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, initial rate analysis Escherichia coli
4.1.3.40 1.4
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, progress curve analysis Escherichia coli
4.1.3.40 1.5
-
 chorismate pH 7.5, wild-type enzyme, initial rate analysis Escherichia coli
4.1.3.40 1.7
-
 chorismate pH 7.5, wild-type enzyme, progress curve analysis Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.40 0.0021
-
 4-hydroxybenzoate pH 7.5, wild-type enzyme Escherichia coli
4.1.3.40 0.26
-
 vanillate pH 7.5, wild-type enzyme Escherichia coli
4.1.3.40 0.33
-
 4-hydroxybenzaldehyde pH 7.5, wild-type enzyme Escherichia coli
4.1.3.40 0.4
-
 3-carboxymethylaminomethyl-4-hydroxybenzoate pH 7.5, wild-type enzyme Escherichia coli