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Literature summary extracted from
- Cloning and expression of rat neutral sphingomyelinase: enzymological characterization and identification of essential histidine residues (2000), Biochim. Biophys. Acta, 1485, 236-246.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | dithiothreitol | approx. 10fold activation at 3-4 mM | Rattus norvegicus |  |
| 3.1.4.12 | Triton X-100 | required for full activity | Rattus norvegicus | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | expression in Escherichia coli | Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | H136A | no activity | Rattus norvegicus |
| 3.1.4.12 | H151A | no activity | Rattus norvegicus |
| 3.1.4.12 | H151Y | no activity | Rattus norvegicus |
| 3.1.4.12 | H272A | no activity | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.12 | 0.0039 | - |
sphingomyelin | 37°C, pH 7.4 | Rattus norvegicus | |
| 3.1.4.12 | 0.048 | - |
1-alkyl-lyso-platelet activating factor | 37°C, pH 7.4 | Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | KCl | 1 M, 3fold activation | Rattus norvegicus | |
| 3.1.4.12 | Mg2+ | absolutely required for activity | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 47600 | - |
x * 47600, deduced from nucleotide sequence | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Rattus norvegicus | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | recombinant nSMase | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | 1-alkyl-lyso-platelet activating factor + H2O | - |
Rattus norvegicus | 1-alkylglycerol + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | ? | x * 47600, deduced from nucleotide sequence | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | neutral sphingomyelinase | - |
Rattus norvegicus |
| 3.1.4.12 | nSMase | - |
Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7 | 7.5 | - |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 5.5 | 8.4 | approx. 25% of maximal actiity at pH 6.0, approx. 75% of maximal activity at pH 8.0 | Rattus norvegicus |
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