EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.2.6 | Y175F | site-directed mutagenesis, mutation has a marginal effect on kcat, but leads to a 8fold increased Km for S-(2-hydroxyacyl)glutathione compared to the wild-type, mutant shows a 30fold increased sensitivity to inhibition by S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione compared to the wild-type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.6 | S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | competitive inhibition | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.6 | 0.2 | - |
S-D-lactoylglutathione | recombinant wild-type enzyme, pH 7.1, 37°C | Homo sapiens | |
3.1.2.6 | 1.6 | - |
S-D-lactoylglutathione | recombinant mutant Y175F, pH 7.1, 37°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.6 | Homo sapiens | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.2.6 | S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate | active site residue Tyr175 contributes to the binding of glutathione derivatives by its hydroxyl group which forms hydrogen binds to the glycine in the glutathione moiety | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.6 | S-D-lactoylglutathione + H2O | - |
Homo sapiens | glutathione + D-lactate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.6 | acetoacetylglutathione hydrolase | - |
Homo sapiens |
3.1.2.6 | Germ cell specific protein | - |
Homo sapiens |
3.1.2.6 | GLO II | - |
Homo sapiens |
3.1.2.6 | glyoxalase II | - |
Homo sapiens |
3.1.2.6 | hydrolase, acetoacetylglutathione | - |
Homo sapiens |
3.1.2.6 | hydrolase, hydroxyacylglutathione | - |
Homo sapiens |
3.1.2.6 | Round spermatid protein RSP29 | - |
Homo sapiens |
3.1.2.6 | S-2-hydroxylacylglutathione hydrolase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.6 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.6 | 510 | - |
S-D-lactoylglutathione | recombinant mutant Y175F, pH 7.1, 37°C | Homo sapiens | |
3.1.2.6 | 780 | - |
S-D-lactoylglutathione | recombinant wild-type enzyme, pH 7.1, 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.6 | 7.1 | - |
assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.6 | 0.0012 | - |
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | recombinant wild-type enzyme, pH 7.1, 37°C | Homo sapiens | |
3.1.2.6 | 0.035 | - |
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | recombinant mutant Y175F, pH 7.1, 37°C | Homo sapiens |