EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | acetyl-CoA | dependent, half maximal activation at 0.014 mM | Mycolicibacterium smegmatis | |
6.4.1.1 | butyryl-CoA | can replace acetyl-CoA with little efficiency | Mycolicibacterium smegmatis | |
6.4.1.1 | propionyl-CoA | can replace acetyl-CoA with little efficiency | Mycolicibacterium smegmatis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.4.1.1 | - |
Mycolicibacterium smegmatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | ADP | - |
Mycolicibacterium smegmatis | |
6.4.1.1 | ATP | - |
Mycolicibacterium smegmatis | |
6.4.1.1 | Co2+ | at excess concentrations | Mycolicibacterium smegmatis | |
6.4.1.1 | L-aspartate | 47% inhibition at 10 mM | Mycolicibacterium smegmatis | |
6.4.1.1 | Mg2+ | at excess concentrations | Mycolicibacterium smegmatis | |
6.4.1.1 | Mn2+ | at excess concentrations | Mycolicibacterium smegmatis | |
6.4.1.1 | additional information | no inhibitors: glutamate and alpha-ketoglutarate | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.1 | 0.2 | - |
ATP | 37°C and 45°C, pH 8, Mg2+ concentration higher than ATP concentration | Mycolicibacterium smegmatis | |
6.4.1.1 | 0.3 | - |
pyruvate | 37°C, pH 8 | Mycolicibacterium smegmatis | |
6.4.1.1 | 0.45 | - |
pyruvate | 45°C, pH 8 | Mycolicibacterium smegmatis | |
6.4.1.1 | 1.26 | - |
ATP | 37°C, pH 8, Mg2+ concentration equal to that of ATP | Mycolicibacterium smegmatis | |
6.4.1.1 | 3 | - |
HCO3- | 37°C, pH 8 | Mycolicibacterium smegmatis | |
6.4.1.1 | 4.6 | - |
HCO3- | 45°C, pH 8 | Mycolicibacterium smegmatis | |
6.4.1.1 | 9.2 | - |
ATP | 45°C, pH 8, Mg2+ concentration equal to that of ATP | Mycolicibacterium smegmatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | Co2+ | can replace Mg2+ with little efficiency | Mycolicibacterium smegmatis | |
6.4.1.1 | Mg2+ | requirement | Mycolicibacterium smegmatis | |
6.4.1.1 | Mn2+ | can replace Mg2+ with little efficiency | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 120000 | - |
x * 120000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | Mycolicibacterium smegmatis | anaplerotic enzyme | ADP + phosphate + oxaloacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.1 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.1 | - |
Mycolicibacterium smegmatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.1 | 150 | - |
- |
Mycolicibacterium smegmatis |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.1 | -70°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol | Mycolicibacterium smegmatis |
6.4.1.1 | 4°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | - |
Mycolicibacterium smegmatis | ADP + phosphate + oxaloacetate | - |
? | |
6.4.1.1 | ATP + pyruvate + HCO3- | anaplerotic enzyme | Mycolicibacterium smegmatis | ADP + phosphate + oxaloacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.1 | multimer | x * 120000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.4.1.1 | PC | - |
Mycolicibacterium smegmatis |
6.4.1.1 | PYC | - |
Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | biotin | - |
Mycolicibacterium smegmatis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.1 | 2.24 | - |
ATP | 45°C, pH 8 | Mycolicibacterium smegmatis |