BRENDA - Enzyme Database show

Agrobacterium tumefaciens beta-glucosidase is also an effective beta-xylosidase, and has a high transglycosylation activity in the presence of alcohols

Watt, D.K.; Ono, H.; Hayashi, K.; Biochim. Biophys. Acta 1385, 78-88 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.126
1-butanol
20 mM, activates due to transglycosylation activity with over 80% of p-nitrophenyl beta-D-glucopyranoside being converted to 1-butyl beta-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol
Agrobacterium tumefaciens
3.2.1.126
1-Heptanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Hexanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Octanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Pentanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-propanol
20 mM, activates, highest rate of consumption of p-nitrophenyl beta-D-glucopyranoside in the presence of 0.6 M 1-propanol
Agrobacterium tumefaciens
Cloned(Commentary)
EC Number
Commentary
Organism
3.2.1.126
expression in Escherichia coli
Agrobacterium tumefaciens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.126
D-glucose
competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
deoxynojirimycin
strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
glucuronolactone
strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
isopropyl 1-thio-beta-D-glucopyranoside
competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
additional information
not inhibited by 24 mM xylose
Agrobacterium tumefaciens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.126
additional information
-
additional information
-
Agrobacterium tumefaciens
3.2.1.126
0.005
-
p-nitrophenyl beta-D-xylopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.012
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.021
-
arbutin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.023
-
coniferin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.045
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.06
-
o-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.08
-
p-nitrophenyl beta-D-fucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.27
-
p-nitrophenyl alpha-L-arabinofuranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
4
-
p-nitrophenyl beta-D-galactopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.126
88289
-
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
3.2.1.126
98000
-
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.126
coniferin + H2O
Agrobacterium tumefaciens
natural substrate
D-glucose + coniferol
-
Agrobacterium tumefaciens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.126
Agrobacterium tumefaciens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.126
recombinant Cbg1
Agrobacterium tumefaciens
Reaction
EC Number
Reaction
Commentary
Organism
3.2.1.126
coniferin + H2O = D-glucose + coniferol
mechanism
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.126
39
41
pH 6.5, 30°C
Agrobacterium tumefaciens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.126
arbutin + H2O
-
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
coniferin + H2O
natural substrate
650284
Agrobacterium tumefaciens
D-glucose + coniferol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
coniferin + H2O
20000fold preference for the natural substrate coniferin over cellobiose
650284
Agrobacterium tumefaciens
D-glucose + coniferol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
additional information
enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
o-nitrophenyl beta-D-glucopyranoside + H2O
-
650284
Agrobacterium tumefaciens
beta-D-glucose + o-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl alpha-L-arabinofuranoside + H2O
with moderate efficiency
650284
Agrobacterium tumefaciens
p-nitrophenol + alpha-L-arabinofuranose
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-cellobioside + H2O
releases only terminal glucose from the substrate
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-fucopyranoside + H2O
with moderate efficiency
650284
Agrobacterium tumefaciens
beta-D-fucose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-galactopyranoside + H2O
with low efficiency
650284
Agrobacterium tumefaciens
beta-D-galactose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-glucopyranoside + H2O
-
650284
Agrobacterium tumefaciens
beta-D-glucose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-xylopyranoside + H2O
73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside
650284
Agrobacterium tumefaciens
beta-D-xylose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
salicin + H2O
-
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.126
?
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.126
30
-
assay at
Agrobacterium tumefaciens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.126
additional information
-
additional information
-
Agrobacterium tumefaciens
3.2.1.126
3.15
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
3.2
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
22.1
-
p-nitrophenyl beta-D-fucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
28.9
-
p-nitrophenyl beta-D-xylopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
46.6
-
p-nitrophenyl beta-D-galactopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
86.7
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
95.4
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
106
-
coniferin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
118
-
p-nitrophenyl alpha-L-arabinofuranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
155
-
o-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.126
6.5
-
assay at
Agrobacterium tumefaciens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.126
0.00078
-
deoxynojirimycin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.0039
-
glucuronolactone
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
2.9
-
D-glucose
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
5.6
-
isopropyl 1-thio-beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.126
Agrobacterium tumefaciens
isoelectric focusing, pH gradient 4-7
-
4.5
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.126
1-butanol
20 mM, activates due to transglycosylation activity with over 80% of p-nitrophenyl beta-D-glucopyranoside being converted to 1-butyl beta-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol
Agrobacterium tumefaciens
3.2.1.126
1-Heptanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Hexanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Octanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-Pentanol
20 mM, activates
Agrobacterium tumefaciens
3.2.1.126
1-propanol
20 mM, activates, highest rate of consumption of p-nitrophenyl beta-D-glucopyranoside in the presence of 0.6 M 1-propanol
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.126
expression in Escherichia coli
Agrobacterium tumefaciens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.126
D-glucose
competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
deoxynojirimycin
strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
glucuronolactone
strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
isopropyl 1-thio-beta-D-glucopyranoside
competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate
Agrobacterium tumefaciens
3.2.1.126
additional information
not inhibited by 24 mM xylose
Agrobacterium tumefaciens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.126
0.00078
-
deoxynojirimycin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.0039
-
glucuronolactone
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
2.9
-
D-glucose
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
5.6
-
isopropyl 1-thio-beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.126
additional information
-
additional information
-
Agrobacterium tumefaciens
3.2.1.126
0.005
-
p-nitrophenyl beta-D-xylopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.012
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.021
-
arbutin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.023
-
coniferin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.045
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.06
-
o-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.08
-
p-nitrophenyl beta-D-fucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
0.27
-
p-nitrophenyl alpha-L-arabinofuranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
4
-
p-nitrophenyl beta-D-galactopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.126
88289
-
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
3.2.1.126
98000
-
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.126
coniferin + H2O
Agrobacterium tumefaciens
natural substrate
D-glucose + coniferol
-
Agrobacterium tumefaciens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.126
recombinant Cbg1
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.126
39
41
pH 6.5, 30°C
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.126
arbutin + H2O
-
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
coniferin + H2O
natural substrate
650284
Agrobacterium tumefaciens
D-glucose + coniferol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
coniferin + H2O
20000fold preference for the natural substrate coniferin over cellobiose
650284
Agrobacterium tumefaciens
D-glucose + coniferol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
additional information
enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
o-nitrophenyl beta-D-glucopyranoside + H2O
-
650284
Agrobacterium tumefaciens
beta-D-glucose + o-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl alpha-L-arabinofuranoside + H2O
with moderate efficiency
650284
Agrobacterium tumefaciens
p-nitrophenol + alpha-L-arabinofuranose
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-cellobioside + H2O
releases only terminal glucose from the substrate
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-fucopyranoside + H2O
with moderate efficiency
650284
Agrobacterium tumefaciens
beta-D-fucose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-galactopyranoside + H2O
with low efficiency
650284
Agrobacterium tumefaciens
beta-D-galactose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-glucopyranoside + H2O
-
650284
Agrobacterium tumefaciens
beta-D-glucose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
p-nitrophenyl beta-D-xylopyranoside + H2O
73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside
650284
Agrobacterium tumefaciens
beta-D-xylose + p-nitrophenol
-
650284
Agrobacterium tumefaciens
?
3.2.1.126
salicin + H2O
-
650284
Agrobacterium tumefaciens
?
-
650284
Agrobacterium tumefaciens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.126
?
x * 98000, about, SDS-PAGE, x * 88289, sequence calculation
Agrobacterium tumefaciens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.126
30
-
assay at
Agrobacterium tumefaciens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.126
additional information
-
additional information
-
Agrobacterium tumefaciens
3.2.1.126
3.15
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
3.2
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
22.1
-
p-nitrophenyl beta-D-fucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
28.9
-
p-nitrophenyl beta-D-xylopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
46.6
-
p-nitrophenyl beta-D-galactopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
86.7
-
Salicin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
95.4
-
p-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
106
-
coniferin
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
118
-
p-nitrophenyl alpha-L-arabinofuranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
3.2.1.126
155
-
o-nitrophenyl beta-D-glucopyranoside
pH 6.5, 30°C
Agrobacterium tumefaciens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.126
6.5
-
assay at
Agrobacterium tumefaciens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.126
Agrobacterium tumefaciens
isoelectric focusing, pH gradient 4-7
-
4.5