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Literature summary extracted from
- Allosteric communication in the tryptophan synthase bienzyme complex: roles of the beta-subunit aspartate 305-arginine 141 salt bridge (2003), Biochemistry, 42, 7807-7818.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | Cs+ | wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 4.2.1.20 | Na+ | wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | NH4+ | wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site | Salmonella enterica subsp. enterica serovar Typhimurium | |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.20 | D305A | forms a bienzyme complex with impaired allosteric communication, altered beta-site substrate reaction specificity, the Na+ form of the alpha-site of the mutant is no longer activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site, but this can be restored by replacing Na+ with NH4+ or Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.20 | R141A | forms a bienzyme complex with impaired allosteric communication, impaired beta-site catalytic acitivity, the Na+ form of the alpha-site of the mutant is no longer activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site, no restoration by NH4+ or Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | DL-alpha-glycerol 3-phosphate | alone or in presence of Na+ and Cs+ for wild-type enzyme, for the mutants R141A and D305A the presence of Na+ and NH4+ reverses the inhibitory effect | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | additional information | - |
additional information | dissociation constants, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.20 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.1.20 | L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), salt bridge betaAsp305-betaArg141 plays a crucial role in both the formation of the closed conformation of the beta-site and the transmission of allosteric signals between the alpha- and beta-sites that switch the alpha-site on and off, mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
| 4.2.1.20 | L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
| 4.2.1.20 | L-serine + indoline | - |
Salmonella enterica subsp. enterica serovar Typhimurium | dihydroiso-L-tryptophan + H2O | i.e. DIT | ? | |
| 4.2.1.20 | additional information | allosteric interactions that regulate substrate channeling and catalysis in the bienzyme complex are triggered by covalent ractions at the beta-site and binding of substrate/product to te alpha-site, transmission of the allosteric signals between the alpha- and beta-catalytic sites is modulated by an ensemble of weak bonding interactions consisting of salt bridges, hydrogen bonds, and van der Waals contacts that switch the subunits between open and closed conformations | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.20 | More | salt-bridges extending between the subunits involve alphaAsp56, betaLys167, beta-Asp305, and betaR141 | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.20 | tetramer | alpha2beta2 enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | additional information | - |
additional information | activities of wild-type and mutants in presence of different effectors | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.033 | - |
indole | beta-reaction, mutant R141A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.16 | - |
1-(indol-3-yl)glycerol 3-phosphate | overall-reaction, mutant R141A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.3 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, wild-type, and overall-reaction, wild-type and mutant D305A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.36 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, mutant R141A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.44 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, mutant D305A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.88 | - |
indole | beta-reaction, mutant D305A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 1.6 | - |
indole | beta-reaction, wild-type, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 6.08 | - |
indole | beta-reaction, mutant D305A, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 7.8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | pyridoxal 5'-phosphate | dependent on | Salmonella enterica subsp. enterica serovar Typhimurium | |
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