EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B17 | ftsH gene, expression as His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B17 | EDTA | - |
Escherichia coli | |
3.4.24.B17 | additional information | no inhibition by phenylmethylsulfonylfluoride | Escherichia coli | |
3.4.24.B17 | o-phenanthroline | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.B17 | additional information | - |
additional information | kinetics | Escherichia coli | |
3.4.24.B17 | 0.021 | - |
ATP | with FGH-NO2FFAF-methoxy, pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 0.023 | - |
protein sigma32 | pH 8.0, 42°C | Escherichia coli | |
3.4.24.B17 | 0.023 | - |
ATP | with protein sigma32, pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 0.104 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 7.3 | - |
GTP | pH 8.0, 37°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.B17 | cytoplasmic membrane | integral, schematic model of structure and arrangement in the membane | Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B17 | Zn2+ | dependent on | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | additional information | - |
the large complexes exhibit either ATPase and protease activity, while smaller ones do not | Escherichia coli |
3.4.24.B17 | 70000 | - |
x * 70000, SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B17 | protein + H2O | Escherichia coli | involved in the degradation of regulatory proteins and uncomplexed subunits of membrane protein complexes | peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B17 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B17 | recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B17 | proteolytic degradation of proteins | mechanism | Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.4.24.B17 | recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity, the refolded enzyme has properties similar to the overexpressed solubilized one | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | 0.0036 | - |
purified recombinant refolded enzyme, substrate protein sigma32 | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B17 | CTP + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.24.B17 | FGH-(NO2)FFAF-methyl ester + H2O | exclusively cleaved at the (NO2)Phe-Phe bond | Escherichia coli | FGH-(NO2)F + Phe-Ala-Phe-methyl ester | - |
? | |
3.4.24.B17 | GTP + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.24.B17 | additional information | large complexes exhibit either ATPase and protease activity, while smaller ones do not | Escherichia coli | ? | - |
? | |
3.4.24.B17 | protein + H2O | - |
Escherichia coli | peptides | - |
? | |
3.4.24.B17 | protein + H2O | involved in the degradation of regulatory proteins and uncomplexed subunits of membrane protein complexes | Escherichia coli | peptides | - |
? | |
3.4.24.B17 | protein SecY + H2O | uncomplexed subunit of the protein translocase | Escherichia coli | ? | - |
? | |
3.4.24.B17 | protein sigma32 + H2O | regulatory protein | Escherichia coli | peptides | approximately 10 peptides with MW below 3 kDa | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.B17 | multimer | x * 70000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B17 | FtsH | - |
Escherichia coli |
3.4.24.B17 | FtsH protease | - |
Escherichia coli |
3.4.24.B17 | M41.001 | Merops-ID | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | 42 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.B17 | 0.003 | - |
protein sigma32 | pH 8.0, 42°C | Escherichia coli | |
3.4.24.B17 | 0.023 | - |
ATP | with FGH-NO2FFAF-methoxy, pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 0.3 | - |
ATP | with protein sigma32, pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 0.32 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 0.53 | - |
GTP | pH 8.0, 37°C | Escherichia coli | |
3.4.24.B17 | 6.08 | - |
GTP | pH 8.0, 37°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B17 | 8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B17 | 2',3'-dideoxy-ATP | - |
Escherichia coli | |
3.4.24.B17 | 2',3'-dideoxy-CTP | - |
Escherichia coli | |
3.4.24.B17 | 2'-deoxy-ATP | - |
Escherichia coli | |
3.4.24.B17 | 3'-deoxy-ATP | - |
Escherichia coli | |
3.4.24.B17 | 7-daza-2'-deoxy-ATP | low activity | Escherichia coli | |
3.4.24.B17 | ATP | - |
Escherichia coli | |
3.4.24.B17 | ATPalphaS | low activity | Escherichia coli | |
3.4.24.B17 | CTP | - |
Escherichia coli | |
3.4.24.B17 | dCTP | - |
Escherichia coli | |
3.4.24.B17 | additional information | no activity with ATPgammaS, absolutely dependent on nucleoside 5'-triphosphates like ATP or CTP, not GTP or UTP, or derivatives | Escherichia coli |