Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tripp, B.C.; Tu, C.; Ferry, J.G.
    Role of the arginine 59 in the gamma-class carbonic anhydrases (2002), Biochemistry, 41, 669-678.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 wild-type and variants expressed in Escherichia coli BL21(DE3) or BL21(DE3) Gold Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 R59A site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59C site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59E site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59H site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59K site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59M site-directed mutagenesis Methanosarcina thermophila
4.2.1.1 R59Q site-directed mutagenesis Methanosarcina thermophila

General Stability

EC Number General Stability Organism
4.2.1.1 freezing and thawing results in formation of aggregates, unfolded at 1.5 M guanidine hydrochloride Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 6.09
-
 CO2 pH 7.5, 37°C, variant R59H, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 6.5
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine Methanosarcina thermophila
4.2.1.1 9.45
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine Methanosarcina thermophila
4.2.1.1 11.7
-
 CO2 pH 7.5, 37°C, variant R59A, 150 mM guanidine Methanosarcina thermophila
4.2.1.1 12.2
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM methylguanidine Methanosarcina thermophila
4.2.1.1 12.8
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine Methanosarcina thermophila
4.2.1.1 13.6
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM guanidine Methanosarcina thermophila
4.2.1.1 14.5
-
 CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
4.2.1.1 15.7
-
 CO2 pH 7.5, 37°C, variant R59A Methanosarcina thermophila
4.2.1.1 16.2
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine Methanosarcina thermophila
4.2.1.1 16.3
-
 CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 16.7
-
 CO2 pH 7.5, 37°C, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 17.1
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM guanidine Methanosarcina thermophila
4.2.1.1 17.3
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM methylguanidine, variant R59Q, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 19.1
-
 CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 19.3
-
 CO2 pH 7.5, 37°C, variant R59E, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 21.8
-
 CO2 pH 7.5, 37°C, wild-type, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 25
-
 CO2 pH 7.5, 37°C, variant R59H, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 25.4
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylamine Methanosarcina thermophila
4.2.1.1 26.2
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM urea Methanosarcina thermophila
4.2.1.1 33.4
-
 CO2 pH 7.5, 37°C, variant R59K, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 33.9
-
 CO2 pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 34
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM imidazole Methanosarcina thermophila
4.2.1.1 34.2
-
 CO2 pH 7.5, 37°C, variant R59C Methanosarcina thermophila
4.2.1.1 43.3
-
 CO2 pH 7.5, 37°C, variant R59M, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 47.6
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine Methanosarcina thermophila
4.2.1.1 51.4
-
 CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ zinc enzyme Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 22873
-
 3 * 22873, measuring A280 value of protein solution Methanosarcina thermophila
4.2.1.1 80000
-
 gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanosarcina thermophila
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanosarcina thermophila P40881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanosarcina thermophila CO2 + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 trimer 3 * 22873, measuring A280 value of protein solution Methanosarcina thermophila

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 Cam
-
 Methanosarcina thermophila
4.2.1.1 carbonic anhydrase
-
 Methanosarcina thermophila
4.2.1.1 gamma-class carbonic anhydrase
-
 Methanosarcina thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 2 8 CO2 pH 7.5, 37°C, variant R59A, 100 mM urea Methanosarcina thermophila
4.2.1.1 10
-
 CO2 pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 12
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine, variant R59H plus 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 13
-
 CO2 pH 7.5, 37°C, variant R59M, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 42
-
 CO2 pH 7.5, 37°C, variant R59H, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 67
-
 CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 70
-
 CO2 pH 7.5, 37°C, variant R59A Methanosarcina thermophila
4.2.1.1 110
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM imidazole Methanosarcina thermophila
4.2.1.1 159
-
 CO2 pH 7.5, 37°C, variant R59M, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 160
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylamine Methanosarcina thermophila
4.2.1.1 310
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine Methanosarcina thermophila
4.2.1.1 320
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine Methanosarcina thermophila
4.2.1.1 350
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine Methanosarcina thermophila
4.2.1.1 520
-
 CO2 pH 7.5, 37°C, variant R59C Methanosarcina thermophila
4.2.1.1 627
-
 CO2 pH 7.5, 37°C, variant R59Q, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 990
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM methylguanidine Methanosarcina thermophila
4.2.1.1 2170
-
 CO2 pH 7.5, 37°C, variant R59C, 100 mM guanidine Methanosarcina thermophila
4.2.1.1 2250
-
 CO2 pH 7.5, 37°C, variant R59A, 150 mM guanidine Methanosarcina thermophila
4.2.1.1 2700
-
 CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
4.2.1.1 2701
-
 CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
4.2.1.1 2740
-
 CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 3270
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM guanidine Methanosarcina thermophila
4.2.1.1 4720
-
 CO2 pH 7.5, 37°C, variant R59K, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 5690
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM methylguanidine Methanosarcina thermophila
4.2.1.1 7140
-
 CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine Methanosarcina thermophila
4.2.1.1 20000
-
 CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 22900
-
 CO2 pH 7.5, 37°C, wild-type, 100 mM guanidine hydrochloride Methanosarcina thermophila
4.2.1.1 62900
-
 CO2 pH 7.5, 37°C, wild-type, without guanidine hydrochloride Methanosarcina thermophila