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Literature summary extracted from
- BetaD305A mutant of tryptophan synthase shows strongly perturbed allosteric regulation and substrate specificity (2001), Biochemistry, 40, 7421-7432.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.20 | D305A | mutation of a salt-bridging residue of the beta-subunit, substitution decreases the affinity of the beta-site for the substrate L-serine, destabilizes the enzyme-bound alpha-aminoacrylate, and quinoid species, and changes the nucleophile specificity of the beta-reaction, increased rate of pyruvate formation compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | alpha-glycerol 3-phosphate | alpha-site substrate analogue, reduces the inducing effect caused by monovalent cation effectors | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 4.2.1.20 | benzimidazole | wild-type and mutant D305A enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | Na+ | inhibits mutant D305A | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | additional information | - |
additional information | kinetic isotope effects with alpha-2H-L-Ser, and thermodynamics, wild-type and mutant | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | Cs+ | activates the wild-type enzyme and mutant D305A | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | K+ | activates the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | Na+ | activates the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | NH4+ | activates the wild-type enzyme and mutant D305A | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.20 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.1.20 | L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
| 4.2.1.20 | L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
| 4.2.1.20 | L-serine + indoline | wild-type and mutant D305A enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | dihydroiso-L-tryptophan | - |
? | |
| 4.2.1.20 | additional information | aniline, phenylhydrazine and methoxylamine react with the wild-type enzyme to form quinoid reaction intermediates, which then decay, enzyme shows conformational changes between the active closed and the inactive open form during substrate recognition and binding, Asp305 of the beta-subunit plays an important role | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.20 | tetramer | alpha2beta2 enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | additional information | - |
additional information | turnover of wild-type and mutant D305A in presence of different effectors for alpha-, and beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.3 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, wild-type enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.4 | - |
L-serine | overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.44 | - |
1-(indol-3-yl)glycerol 3-phosphate | alpha-reaction, mutant D305A enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.8 | - |
L-serine | overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of K+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 0.88 | - |
L-serine | beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 1.4 | - |
indoline | overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of 100 mM Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 1.6 | - |
L-serine | beta-reaction, wild-type enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 2 | - |
L-serine | overall-reaction, wild-type enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 5 | - |
L-serine | overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of NH4+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 6.08 | - |
L-serine | beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 7.8 | - |
L-serine | overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 8 | - |
L-serine | overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 10.3 | - |
L-serine | overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of NH4+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 12 | - |
L-serine | overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of K+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.2.1.20 | 14 | - |
L-serine | overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 7.8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
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