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Literature summary extracted from

  • Tripp, B.C.; Ferry, J.G.
    A structure-function study of a proton transport pathway in teh gamma-class carbonic anhydrase from Methanosarcina thermophila (2000), Biochemistry, 39, 9232-9240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli BL21(DE3) or Bl21(DE3) Gold Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 E62A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62A/E84A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62C site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62D site-directed mutagenesis, 3fold decrease in kcat Methanosarcina thermophila
4.2.1.1 E62D/E84D site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62H site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62Q site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62T site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E62Y site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E84 A site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 C site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 D site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 H site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 K site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 Q site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 S site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E84 Y site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
4.2.1.1 E88A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
4.2.1.1 E89A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 0.8
-
 CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 1.1
-
 CO2 pH 7.5, 25°C, variant E84C, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1.2
-
 CO2 pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1.4
-
 CO2 pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1.5
-
 CO2 pH 7.5, 25°C, variant E84A and E84K, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 2.1
-
 CO2 pH 7.5, 25°C, variant E84S and E84Y, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 2.5
-
 CO2 pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 2.9
-
 CO2 pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 3
-
 CO2 pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 3.2
-
 CO2 pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 3.8
-
 CO2 pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 5.4
-
 CO2 pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 5.5
-
 CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 6.5
-
 CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 7.6
-
 CO2 pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 8.8
-
 CO2 pH 7.5, 25°C, variant E84A, 50 mM IMID buffer Methanosarcina thermophila
4.2.1.1 9.4
-
 CO2 pH 7.5, 25°C, variant E62A, 50 mM IMID buffer Methanosarcina thermophila
4.2.1.1 12.6
-
 CO2 pH 8.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 14.7
-
 CO2 pH 7.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 16.9
-
 CO2 pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 18.1
-
 CO2 pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 18.8
-
 CO2 pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 19.3
-
 CO2 pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 20
-
 CO2 pH 8.5, 25°C, wild-type, H2O Methanosarcina thermophila
4.2.1.1 22.5
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 23.8
-
 CO2 pH 6.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 27.8
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 28.5
-
 CO2 pH 6.5, 25°C, wild-type Methanosarcina thermophila
4.2.1.1 44.1
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM IMID buffer Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+
-
 Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 22873
-
 3 * 22873, determined by measuring A280S of protein solution Methanosarcina thermophila
4.2.1.1 70000
-
 gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanosarcina thermophila
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanosarcina thermophila CO2 + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 trimer 3 * 22873, determined by measuring A280S of protein solution Methanosarcina thermophila

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 Cam
-
 Methanosarcina thermophila
4.2.1.1 carbonic anhydrase
-
 Methanosarcina thermophila
4.2.1.1 gamma-class carbonic anhydrase
-
 Methanosarcina thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 47
-
 CO2 pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 210
-
 CO2 pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 340
-
 CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 410
-
 CO2 pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 510
-
 CO2 pH 7.5, 25°C, variant E84Y, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 720
-
 CO2 pH 7.5, 25°C, variant E84C and E62A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 800
-
 CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 860
-
 CO2 pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 870
-
 CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 910
-
 CO2 pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1050
-
 CO2 pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1070
-
 CO2 pH 7.5, 25°C, variant E62A, 50 mM IMID buffer Methanosarcina thermophila
4.2.1.1 1080
-
 CO2 pH 7.5, 25°C, variant E84S, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1300
-
 CO2 pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 1900
-
 CO2 pH 7.5, 25°C, variant E84K, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 4700
-
 CO2 pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 7100
-
 CO2 pH 5.7-9.5, 25°C, wild-type Methanosarcina thermophila
4.2.1.1 9300
-
 CO2 pH 6.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 9500
-
 CO2 pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 16700
-
 CO2 pH 7.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 16800
-
 CO2 pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 18600
-
 CO2 pH 6.5, 25°C, wild-type Methanosarcina thermophila
4.2.1.1 21000
-
 CO2 pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 22600
-
 CO2 pH 8.5, 25°C, wild-type, D2O Methanosarcina thermophila
4.2.1.1 24700
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer Methanosarcina thermophila
4.2.1.1 26500
-
 CO2 pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 29100
-
 CO2 pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 35100
-
 CO2 pH 7.5, 25°C, variant E84A, 50 mM IMID buffer Methanosarcina thermophila
4.2.1.1 56800
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS buffer Methanosarcina thermophila
4.2.1.1 58900
-
 CO2 pH 8.5, 25°C, wild-type Methanosarcina thermophila
4.2.1.1 71900
-
 CO2 pH 7.5, 25°C, wild-type, 50 mM IMID buffer Methanosarcina thermophila