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Literature summary extracted from

  • Monnaie, D.; Arosio, D.; Griffon, N.; Rose, T.; Rezaie, A.R.; Di Cera, E.
    Identification of a binding site for quaternary amines in factor Xa (2000), Biochemistry, 39, 5349-5354.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.6 K+ slightly activating Homo sapiens
3.4.21.6 additional information not affected by NH4+ and Li+ Homo sapiens
3.4.21.6 Na+ 4fold activation at 150 mM Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.6 F174N site-directed mutagenesis, reduced binding of inhibitor trimethylphenylammonium Homo sapiens
3.4.21.6 Y99T site-directed mutagenesis, 25fold reduced binding of inhibitor trimethylphenylammonium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.6 4-aminobenzamidine competitive Homo sapiens
3.4.21.6 choline positively linked to Na+ binding Homo sapiens
3.4.21.6 additional information not affected by NH4+ and Li+ Homo sapiens
3.4.21.6 trimethylphenylammonium i.e. TMPA, strong and highly specific inhibition, inhibits substrate hydrolysis competitively, does not inhibit the binding of 4-aminobenzamidine to the S1-pocket, and is positively linked to Na+ binding rsulting in 10fold increase in binding of inhibitor at 0.15 M Na+, interacts with Y99, F174, and W215 via van der Waals interactions, and the charged amine coupling electrostatically with the carboxylates of E97 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.6 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.6 selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.6 L-Arg-Gly-Arg-4-nitroanilide + H2O chromogenic substrate Homo sapiens L-Arg-Gly-Arg + 4-nitroaniline
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.6 More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.6 25
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.6 8
-
assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.6 additional information
-
additional information inhibition kinetics Homo sapiens
3.4.21.6 0.085
-
4-aminobenzamidine pH 8.0, 25°C, with wild-type enzyme Homo sapiens
3.4.21.6 5
-
trimethylphenylammonium pH 8.0, 25°C, with wild-type enzyme Homo sapiens
3.4.21.6 20
-
trimethylphenylammonium pH 8.0, 25°C, with mutant F174N enzyme Homo sapiens
3.4.21.6 107
-
trimethylphenylammonium pH 8.0, 25°C, with mutant Y99T enzyme Homo sapiens