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Literature summary extracted from

  • Fan, Y.X.; McPhie, P.; Miles, E.W.
    Regulation of tryptophan synthase by temperature, monovalent cations, and an allosteric ligand. Evidence from Arrhenius plots, absorption spectra, and primary kinetic isotope effects (2000), Biochemistry, 39, 4692-4703.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.20 guanidinium i.e. GuH+, involved in the thermal stability and activity equilibrium of the enzyme complex, overview Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 additional information
-
 additional information kinetics and temperature-dependence Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.20 Cs+ involved in the thermal stability and activity equilibrium of the enzyme complex, overview Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 Na+ involved in the thermal stability and activity equilibrium of the enzyme complex, overview Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.20 190
-
 wild-type enzyme, in presence of guanidinum Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 1310
-
 wild-type enzyme, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 1400
-
 wild-type enzyme, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 additional information the intermediate product indole is transferred from the alpha- to the beta-site through a 25 A long hydrophobic tunnel Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 More structure and conformation regulating the activity and allosteric communication in the enzyme complex, modeling of the high activity closed form and the low activity open form dependent on temperature, conversion from the open to the closed form at high temperature Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.20 5 50 thermodynamic analysis of the conformational change conditions and the effects of monovalent ions and effector DL-alpha-glycderol 3-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.20 7.8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium