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Literature summary extracted from

  • Jhee, K.H.; McPhie, P.; Miles, E.W.
    Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation (2000), Biochemistry, 39, 10548-10556.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.22
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 additional information truncated version residues 1-353 is catalytically active and binds pyridoxal phosphate, removal of residues 354-507 increases the specific activity and alters steady-state kinetic parameters Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 2.2
-
 L-serine 37°C Saccharomyces cerevisiae
4.2.1.22 2.2
-
 homocysteine 37°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 70000
-
 gel filtration, truncated enzyme, dimer Saccharomyces cerevisiae
4.2.1.22 400000
-
 gel filtration, truncated enzyme, octamer Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.22
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.22 73
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-Serine + homocysteine
-
 Saccharomyces cerevisiae Cystathionine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.22 dimer truncated enzyme Saccharomyces cerevisiae
4.2.1.22 octamer full-length enzyme is a mixture of tetramer and octamer Saccharomyces cerevisiae
4.2.1.22 tetramer full-length enzyme is a mixture of tetramer and octamer Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.22 7.6
-
 L-serine 37°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.22 8.5
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 pyridoxal 5'-phosphate dependent Saccharomyces cerevisiae