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Literature summary extracted from

  • Kiema, T.R.; Engel, C.K.; Schmitz, W.; Filppula, S.A.; Wierenga, R.K.; Hiltunen, J.K.
    Mutagenic and enzymological studies of the hydratase and isomerase activities of 2-enoyl-CoA hydratase-1 (1999), Biochemistry, 38, 2991-2999.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.17 expressed in Escherichia coli BL21 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.17 E144A kcat for trans-2-hexenoyl-CoA is 1733fold lower than wild-type value Rattus norvegicus
4.2.1.17 E144A/Q162L kcat for trans-2-hexenoyl-CoA is 12417fold lower than wild-type value. The point mutations E144A and Q162L by themselves apparently do not cause structural rearrangements of the active site helix, but when both residues are changed, the active site geometry changes Rattus norvegicus
4.2.1.17 E164A kcat for trans-2-hexenoyl-CoA is 1709fold lower than wild-type value Rattus norvegicus
4.2.1.17 Q162A kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus
4.2.1.17 Q162L kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus
4.2.1.17 Q162M kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.17 0.0025
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
4.2.1.17 0.0029
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
4.2.1.17 0.003
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
4.2.1.17 0.0039
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
4.2.1.17 0.005
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
4.2.1.17 0.0052
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
4.2.1.17 0.0063
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
4.2.1.17 0.0143
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
4.2.1.17 0.0152
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
4.2.1.17 0.021
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
4.2.1.17 0.0229
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
4.2.1.17 0.024
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
4.2.1.17 0.025
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
4.2.1.17 0.027
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
4.2.1.17 0.0499
-
 crotonyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.17 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.17 recombinant enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.17 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.17 64
-
 homogenate of cells transformed with the pET3aECH1 expression vector Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 crotonyl-CoA + H2O
-
 Rattus norvegicus (3S)-3-hydroxybutanoyl-CoA
-
 ?
4.2.1.17 additional information the enzyme also catalyzes DELTA3-DELTA2-isomerization of trans-3-hexenoyl-CoA Rattus norvegicus ?
-
 ?
4.2.1.17 trans-2-decenoyl-CoA + H2O
-
 Rattus norvegicus (3S)-hydroxydecanoyl-CoA
-
 ?
4.2.1.17 trans-2-hexenoyl-CoA + H2O
-
 Rattus norvegicus (3S)-3-hydroxyhexanoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 2-enoyl-CoA hydratase-1
-
 Rattus norvegicus
4.2.1.17 crotonase
-
 Rattus norvegicus
4.2.1.17 mECH-1
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.17 22
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.17 0.0121
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
4.2.1.17 0.06
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A/Q162L Rattus norvegicus
4.2.1.17 0.085
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
4.2.1.17 0.095
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
4.2.1.17 0.43
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E144A Rattus norvegicus
4.2.1.17 0.44
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme E164A Rattus norvegicus
4.2.1.17 104
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
4.2.1.17 164
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
4.2.1.17 174
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
4.2.1.17 203
-
 trans-2-decenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
4.2.1.17 561
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162L Rattus norvegicus
4.2.1.17 601
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162M Rattus norvegicus
4.2.1.17 607
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, mutant enzyme Q162A Rattus norvegicus
4.2.1.17 745
-
 trans-2-hexenoyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus
4.2.1.17 2238
-
 crotonyl-CoA pH 8.0, 22°C, wild-type enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.17 8
-
 assay at Rattus norvegicus