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Literature summary extracted from

  • Weyand, M.; Schlichting, I.
    Crystal structure of wild-type tryptophan synthase complexed with the natural substrate indole-3-glycerol phosphate (1999), Biochemistry, 38, 16469-16480.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.20 purified recombinant wild-type enzyme alone or in complex with alpha-subunit substrate analogue indole propanol phosphate, 10 mg/ml in 50 mM bicine, pH 7.8, 10 mM Na-EDTA, 0.02 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, in the dark at room temperature, hanging drop method, equal volumes of protein and reservoir solution, the latter containing 50 mM bicine, pH 7.8, 5 mM dithioerytritol, 5 mM Na-EDTA, 0.1 mM pyridoxal 5'-phosphate, 2 mM spermine, 2 mM NaN3, and 8-12% w/v PEG 8000, with or without 7 mM indole propanol phosphate, X-ray diffraction structure determination and analysis at 1.4 A resolution, structure modeling Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 additional information the intermediate product indole is transferred from the alpha- to the beta-site through a 25 A long hydrophobic tunnel Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 TRPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium