EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.1 | cam gene expressed in Escherichia coli | Methanosarcina thermophila |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.1.1 | - |
Methanosarcina thermophila |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.1 | Co2+ | isomorphously replaces zinc | Methanosarcina thermophila | |
4.2.1.1 | Zn2+ | - |
Methanosarcina thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.1 | 70000 | - |
apo-Cam and Co-Cam, gel filtration | Methanosarcina thermophila |
4.2.1.1 | 74000 | - |
Cam and Zn-Cam, gel filtration | Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.1 | H2CO3 | Methanosarcina thermophila | - |
CO2 + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.1 | Methanosarcina thermophila | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.1 | recombinant enzyme | Methanosarcina thermophila |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.1 | additional information | - |
specific activity Cam 720 units/mg, apo-Cam 20 units/mg, Co-Cam 2020 units/mg, Zn-Cam 910 units/mg, one unit = (t0-t)/t where t0 is the time for the uncatalyzed reaction and t is the time for the catalyzed reaction | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.1 | H2CO3 | - |
Methanosarcina thermophila | CO2 + H2O | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.1 | gamma-carbonic anhydrase | - |
Methanosarcina thermophila |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.1 | 0.00061 | - |
CO2 | pH 5.5-7.5, 25°C, Zn-Cam | Methanosarcina thermophila | |
4.2.1.1 | 0.00104 | - |
CO2 | pH 5.5-7.5, 25°C, Co-Cam | Methanosarcina thermophila |