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Literature summary extracted from

  • MacBeath, G.; Kast, P.; Hilvert, D.
    A small, thermostable, and monofunctional chorismate mutase from the archaeon Methanococcus jannaschii (1998), Biochemistry, 37, 10062-10073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expression in Escherichia coli Methanocaldococcus jannaschii
5.4.99.5 expression in Escherichia coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.041
-
chorismate 30°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 25790
-
gel filtration Escherichia coli
5.4.99.5 29390
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Methanocaldococcus jannaschii biosynthesis of aromatic amino acids Prephenate
-
?
5.4.99.5 Chorismate Escherichia coli biosynthesis of aromatic amino acids Prephenate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Escherichia coli P0A9J8
-
-
5.4.99.5 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
Methanocaldococcus jannaschii
5.4.99.5 N-terminal Met1 is in most samples removed Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
5.4.99.5 misfolded, higher-order aggregates convert into fully active dimeric protein by denaturation with guanidinium chloride and subsequent renaturation upon dilution into native buffer Escherichia coli
5.4.99.5 refolding after treatment with guanidinium chloride Methanocaldococcus jannaschii
5.4.99.5 refolding after treatment with urea Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
Methanocaldococcus jannaschii Prephenate
-
?
5.4.99.5 Chorismate
-
Escherichia coli Prephenate
-
?
5.4.99.5 Chorismate biosynthesis of aromatic amino acids Methanocaldococcus jannaschii Prephenate
-
?
5.4.99.5 Chorismate biosynthesis of aromatic amino acids Escherichia coli Prephenate
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 homodimer alpha2, 2 * 11883-11887, deduced from amino acid sequence, ESI-MS, MALDI-MS Escherichia coli
5.4.99.5 homodimer alpha2, 2 * 12843-12848, deduced from amino acid sequence, ESI-MS, MALDI-MS Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 chorismate mutase
-
Methanocaldococcus jannaschii
5.4.99.5 chorismate mutase
-
Escherichia coli
5.4.99.5 EcCM
-
Escherichia coli
5.4.99.5 MjCM
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.5 63
-
midpoint of unfolding transitions Escherichia coli
5.4.99.5 88
-
midpoint of unfolding transitions Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 5.7
-
chorismate 30°C Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.5 5 9
-
Methanocaldococcus jannaschii