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Literature summary extracted from
- DNA sequence of butyrylcholinesterase from the rat: expression of the protein and characterization of the properties of rat butyrylcholinesterase (2002), Biochem. Pharmacol., 63, 2101-2110.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.8 | expressed in CHO cells | Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.8 | R286L | Km values similar to wild-type enzyme | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.7 | 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide | i.e. BW284c51, complete inhibition | Rattus norvegicus |  |
| 3.1.1.8 | Echothiophate | enzyme spontaneously reactivates with a half-life of 4.3 h | Rattus norvegicus | |
| 3.1.1.8 | Triton X-100 | competitive inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.8 | 0.037 | - |
acetylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 3.1.1.8 | 0.061 | - |
acetylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
| 3.1.1.8 | 0.094 | - |
butyrylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 3.1.1.8 | 0.112 | - |
propionylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 3.1.1.8 | 0.134 | - |
butyrylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
| 3.1.1.8 | 0.189 | - |
propionylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.7 | Rattus norvegicus | - |
- |
- |
| 3.1.1.8 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.8 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.7 | serum | - |
Rattus norvegicus | - |
| 3.1.1.8 | serum | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.7 | acetylthiocholine + H2O | best substrate | Rattus norvegicus | thiocholine + acetate | - |
? | |
| 3.1.1.8 | acetylthiocholine + H2O | - |
Rattus norvegicus | thiocholine + acetate | - |
? | |
| 3.1.1.8 | benzoylcholine + H2O | - |
Rattus norvegicus | choline + benzoate | - |
? | |
| 3.1.1.8 | bytyrylhiocholine + H2O | - |
Rattus norvegicus | thiocholine + butyrate | - |
? | |
| 3.1.1.8 | cocaine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.1.1.8 | propionylthiocholine + H2O | best substrate | Rattus norvegicus | thiocholine + propionate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.8 | 3350 | - |
propionylthiocholine | pH 7.0, 25°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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