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Literature summary extracted from
- Purification, separation and characterization of phosphoglucomutase and phosphomannomutase from maize leaves (1998), Biochem. Mol. Biol. Int., 46, 461-470.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.2 | D-glucose-1,6-bisphosphate | - |
Zea mays |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.2.8 | higly unstable during storage | Zea mays |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.2 | D-Glucose 1,6-bisphosphate | - |
Zea mays | |
| 5.4.2.2 | D-glucose 1-phosphate | - |
Zea mays | |
| 5.4.2.2 | D-mannose 1-phosphate | - |
Zea mays | |
| 5.4.2.2 | Mn2+ | approx. 50% inhibition at 5 mM, almost complete inhibition at 7 mM | Zea mays | |
| 5.4.2.8 | Mn2+ | complete inhibition above 2 mM | Zea mays | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.2 | 0.016 | - |
alpha-D-Glucose 1,6-bisphosphate | 25°C, pH 7.6 | Zea mays | |
| 5.4.2.2 | 0.02 | - |
alpha-D-glucose 1-phosphate | 25°C, pH 7.6 | Zea mays | |
| 5.4.2.2 | 0.02 | - |
D-mannose 1-phosphate | 25°C, pH 7.6 | Zea mays | |
| 5.4.2.8 | 1.37 | - |
alpha-D-mannose 1-phosphate | - |
Zea mays | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.2 | Mg2+ | approx. 4fold activation at 4 mM | Zea mays | |
| 5.4.2.8 | Mg2+ | approx. 3fold activation at 1 mM-5 mM | Zea mays | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 66000 | - |
2 * 66000, SDS-PAGE | Zea mays |
| 5.4.2.2 | 132000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.2 | alpha-D-glucose 1-phosphate | Zea mays | - |
alpha-D-glucose 6-phosphate | - |
r | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | Zea mays | - |
alpha-D-mannose 6-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.2 | Zea mays | - |
- |
- |
| 5.4.2.8 | Zea mays | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.2 | ammonium sulfate, Sephadex G-25, DEAE-Toyopearl, Toyopearl HW-65 | Zea mays |
| 5.4.2.8 | ammonium sulfate, Sephadex G-25, KM-cellulose, partially purified | Zea mays |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 11.7 | - |
- |
Zea mays |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.2.2 | 0-4°C, 50% glycerol, 2-3 d, no loss of activity | Zea mays |
| 5.4.2.8 | 0-4°C, 50% glycerol, 12 h, 70% loss of activity | Zea mays |
| 5.4.2.8 | 0-4°C, 50% glycerol, 3-4 d, complete loss of activity | Zea mays |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.2 | alpha-D-glucose 1,6-bisphosphate + H2O | - |
Zea mays | alpha-D-glucose 6-phosphate + phosphate | - |
? | |
| 5.4.2.2 | alpha-D-glucose 1-phosphate | - |
Zea mays | alpha-D-glucose 6-phosphate | - |
r | |
| 5.4.2.2 | alpha-D-glucose 1-phosphate | - |
Zea mays | D-glucose 6-phosphate | - |
? | |
| 5.4.2.2 | alpha-D-mannose 1-phosphate | - |
Zea mays | alpha-D-mannose 6-phosphate | - |
r | |
| 5.4.2.2 | D-Mannose 1-phosphate | - |
Zea mays | D-Mannose 6-phosphate | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Zea mays | alpha-D-mannose 6-phosphate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | dimer | 2 * 66000, SDS-PAGE | Zea mays |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.2 | PGM | - |
Zea mays |
| 5.4.2.8 | PMM | - |
Zea mays |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 50 | - |
no loss of activity after 3 min | Zea mays |
| 5.4.2.8 | 50 | - |
40% loss of activity after 3 min | Zea mays |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.2 | 8 | - |
- |
Zea mays |
| 5.4.2.8 | 7.4 | - |
- |
Zea mays |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.2 | 0.23 | - |
D-Glucose 1,6-bisphosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
| 5.4.2.2 | 1.35 | - |
D-mannose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
| 5.4.2.2 | 3.3 | - |
D-glucose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
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