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Literature summary extracted from

  • Nibeilliu, M.E.; Malthouse, J.P.
    The stereospecificity and catalytic efficiency of the tryptophan synthase catalysed exchange of the alpha-protons of amino acids (2004), Biochem. J., 381, 847-852.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 coexpression of trpA and trp, encoding the alpha- and beta-subunit of the enzyme in Escherichia coli strain CB149 lacking the trp operon Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 additional information
-
 additional information kinetics of the hydrogen-deuterium exchange reactions Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 recombinant alpha- and beta-subunits coexpressed in Escherichia coli, separation of alpha-subunit from beta-subunit by acid precipitation of the beta-subunit at pH 4.5, separation beta-subunit from alpha-subunit by heat denaturation of the alpha-subunit at 63°C and pH 7.8 Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate
-
 ?
4.2.1.20 additional information the enzyme catalyzes the hydrogen-deuterium exchange of the pro-2R and pro-2S protons of [2-13C]glycine at pH 7.8, as well as the hydrogen-deuterium exchange of the alpha-proton of a range of L- and D-amino acids at pH 7.8, with the exception of tryptophan and norleucine, the stereospecificities of the first-order alpha-proton exchange rates are independent of the size and electronegativity of the mino acid R-group, similar second-order proteon exchnage rates with L-tryptophan and L-serine showing especially high stereospecificity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.20 tetramer alpha2beta2 enzyme complex Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.20 7.8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium