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Literature summary extracted from

  • Fini, C.; Talamo, F.; Cherri, S.; Coli, M.; Floridi, A.; Ferrara, L.; Scaloni, A.
    Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma (2003), Biochem. J., 372, 443-451.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 alpha,beta-methylene-adenosine 5'-diphosphate inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 Bos taurus
3.1.3.5 ATP inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 Bos taurus

Application

EC Number Application Comment Organism
3.1.3.5 medicine target for a number of therapeutic drugs since increased levels of the enzyme correlate with various disease states Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.03
-
 dCMP isoenzyme 5'-NT-3 Bos taurus
3.1.3.5 0.04
-
 dCMP isoenzyme 5'-NT-2 Bos taurus
3.1.3.5 0.05
-
 dCMP isoenzyme 5'-NT-1 Bos taurus
3.1.3.5 0.06
-
 5'-AMP isoenzyme 5'-NT-2 and 5'-NT-4 Bos taurus
3.1.3.5 0.06
-
 5'-IMP isoenzyme 5'-NT-3 Bos taurus
3.1.3.5 0.06
-
 dCMP isoenzyme 5'-NT-4 Bos taurus
3.1.3.5 0.07
-
 5'-IMP isoenzyme 5'-NT-2 and 5'-NT-4 Bos taurus
3.1.3.5 0.07
-
 5'-GMP isoenzyme 5'-NT-4 Bos taurus
3.1.3.5 0.09
-
 5'-GMP isoenzyme 5'-NT-2 Bos taurus
3.1.3.5 0.11
-
 5'-AMP isoenzyme 5'-NT-1 and 5'-NT-3 Bos taurus
3.1.3.5 0.15
-
 5'-IMP isoenzyme 5'-NT-1 Bos taurus
3.1.3.5 0.16
-
 5'-GMP isoenzyme 5'-NT-3 Bos taurus
3.1.3.5 0.22
-
 5'-GMP isoenzyme 5'-NT-1 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 membrane glycosylphosphatidyl-inositol-anchored Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 66200
-
 x * 66200, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.5 additional information Bos taurus the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 glycoprotein differential glycosylation, especially in the oligosaccharides linked to Asn403, an increase in sialylated glycans, together with a decrease in high-mannose structures is observed arising from 5'-NT-1 to 5'-NT-4 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 seminal plasma
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 300
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Bos taurus adenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Bos taurus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Bos taurus inosine + phosphate
-
 ?
3.1.3.5 dCMP + H2O
-
 Bos taurus deoxycytidine + phosphate
-
 ?
3.1.3.5 additional information significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site Bos taurus ?
-
 ?
3.1.3.5 additional information the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 66200, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 5'-NT-1
-
 Bos taurus
3.1.3.5 5'-NT-2
-
 Bos taurus
3.1.3.5 5'-NT-3
-
 Bos taurus
3.1.3.5 5'-NT-4
-
 Bos taurus
3.1.3.5 ecto-5'-NT
-
 Bos taurus
3.1.3.5 ecto-5'-nucleotidase
-
 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.5 Bos taurus isoenzyme 5'-NT-4
-
 6.1
3.1.3.5 Bos taurus isoenzyme 5'-NT-3
-
 6.2
3.1.3.5 Bos taurus isoenzyme 5'-NT-2 6.8 6.3
3.1.3.5 Bos taurus isoenzyme 5'-NT-1
-
 6.5