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Literature summary extracted from

  • Tsoukatos, D.C.; Liapikos, T.A.; Tselepis, A.D.; Chapman, M.J.; Ninio, E.
    Platelet-activating factor acetylhydrolase and transacetylase activities in human plasma low-density lipoprotein (2001), Biochem. J., 357, 457-464.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.149 4-(2-aminoethyl)benzene-sulfonyl fluoride i.e. Pefabloc, complete inhibition of both enzyme activities at 0.05 mM Homo sapiens
2.3.1.149 diisopropylfluorophosphate complete inhibition of both enzyme activities at 20 mM Homo sapiens
2.3.1.149 ether/ester-linked lysophospholipids competitive Homo sapiens
3.1.1.47 1-O-hexadecanoyl-sn-glycero-3-phosphocholine competitive inhibition of acetylhydrolase activity Homo sapiens
3.1.1.47 4-(2-aminoethyl)benzenesulfonyl fluoride i.e. pefabloc, inhibits both acetylhydrolase and transacetylase activities completely at 0.05 mM for the native enzyme, and at 1 mM for the recombinant enzyme Homo sapiens
3.1.1.47 diisopropylfluorophosphate inhibits both acetylhydrolase and transacetylase activities completely at 20 mM for the native and the recombinant enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.47 additional information
-
 additional information the recombinant enzyme shows allosteric kinetics in the transacetylation reaction Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.149 LDL particle isolated from human plasma, different subfractions Homo sapiens
-
-
3.1.1.47 extracellular secretion, plasma Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.149 Cu2+ enzyme activities are decreased upon oxidation of LDL with Cu2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.149 additional information Homo sapiens the enzyme transfers short-chain fatty acids from platelet-activating factor PAF and its close ether- and ester-linked analogues to ether/ester-linked lysophospholipids, enzyme activity distribution within different LDL-subfactions, overview ?
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine Homo sapiens transacetylation 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine Homo sapiens the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. platelet-activation factor, biological inactivation by deacetylation 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 additional information Homo sapiens the enzyme is associated to low-density lipoprotein LDL particles, both enzyme activities, acetylhydrolase and transacetylase, decrease during LDL oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.149 Homo sapiens
-
-
-
3.1.1.47 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.149 enzyme activities are decreased upon oxidation of LDL with Cu2+ Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.47 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate the enzyme possesses both acetylhydrolase and transacetylase activities which remove PAF and its ether-linked analogues from LDL-particles upon LDL oxidation Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.149 commercial preparation purified recombinant enzyme Homo sapiens
-
3.1.1.47 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.149 additional information
-
 activities of different LDL subfractions Homo sapiens
3.1.1.47 0.015
-
 transacetylase activity Homo sapiens
3.1.1.47 0.022
-
 acetylhydrolase activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.149 1-acyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid 1-acyl-sn-glycero-3-phosphocholine is termed lyso-PC Homo sapiens 1-organyl-sn-glycero-3-phosphocholine + 1-acyl-2-lyso-sn-glycero-3-phospholipid
-
 ?
2.3.1.149 1-hexadecanoyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid 1-hexadecanoyl-sn-glycero-3-phosphocholine is termed C16:0 lyso-PC Homo sapiens 1-organyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-2-lyso-sn-glycero-3-phospholipid
-
 ?
2.3.1.149 1-O-alkyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid 1-O-alkyl-sn-glycero-3-phosphocholine is termed lyso-PAF, artificial substrate Homo sapiens 1-organyl-sn-glycero-3-phosphocholine + 1-O-alkyl-2-lyso-sn-glycero-3-phospholipid
-
 ?
2.3.1.149 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine is termed C16:0 lyso-PAF Homo sapiens 1-organyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-lyso-sn-glycero-3-phospholipid
-
 ?
2.3.1.149 additional information the enzyme transfers short-chain fatty acids from platelet-activating factor PAF and its close ether- and ester-linked analogues to ether/ester-linked lysophospholipids, enzyme activity distribution within different LDL-subfactions, overview Homo sapiens ?
-
 ?
2.3.1.149 additional information the enzyme also has a platelet-activating factor acetylhydrolase activity Homo sapiens ?
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine transacetylase activity Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine transacetylation Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine transacetylase activity Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor PAF Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor, biological inactivation by deacetylation Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine transacetylase activity, 40% of the activity with PAF Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine transacetylase activity, 73% of the activity with PAF Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine transacetylase activity, 17% of the activity with PAF Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + 2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 additional information cholesteryl acetate is inactive as acetyl donor in the transacetylation reaction Homo sapiens ?
-
 ?
3.1.1.47 additional information the enzyme is associated to low-density lipoprotein LDL particles, both enzyme activities, acetylhydrolase and transacetylase, decrease during LDL oxidation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.149 platelet-activating factor transacetylase
-
 Homo sapiens
3.1.1.47 PAF acetylhydrolase
-
 Homo sapiens
3.1.1.47 PAF-AH
-
 Homo sapiens
3.1.1.47 platelet-activating factor acetylhydrolase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.149 37
-
 assay at Homo sapiens
3.1.1.47 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.149 60
-
 60 min, loss of 10% transacetylase activity, acetylhydrolase activity is completely stable Homo sapiens
3.1.1.47 60
-
 60 min, complete inactivation of both activities, acetylhydrolase and transacetylase Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.149 7.4
-
 assay at Homo sapiens
3.1.1.47 7.4
-
 assay at Homo sapiens