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Literature summary extracted from

  • Genschel, U.; Powell, C.A.; Abell, C.; Smith, A.G.
    The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice) (1999), Biochem. J., 341, 669-678.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1
-
 Oryza sativa
6.3.2.1 expression Escherichia coli Lotus japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.044
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus
6.3.2.1 0.44
-
 beta-Alanine 25°C, pH 8.0, at 20 mM pantoate Lotus japonicus
6.3.2.1 0.98
-
 beta-Alanine 25°C, pH 8.0, at 0.5 mM pantoate Lotus japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.1 cytosol
-
 Lotus japonicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.1 33900
-
 x * 33900, deduced from nucleotide sequence Oryza sativa
6.3.2.1 34000
-
 2 * 34000, SDS-PAGE Lotus japonicus
6.3.2.1 72800
-
 gel filtration Lotus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine Lotus japonicus
-
 AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine Oryza sativa
-
 AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Lotus japonicus O24035
-
-
6.3.2.1 Oryza sativa O24210
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1 recombinant pantothenate snthetase Lotus japonicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.1 1.36
-
-
 Lotus japonicus

Storage Stability

EC Number Storage Stability Organism
6.3.2.1 -20°C, no loss of activity Lotus japonicus
6.3.2.1 4°C, 4 weeks, 25% loss of activity Lotus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Lotus japonicus AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Oryza sativa AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 ? x * 33900, deduced from nucleotide sequence Oryza sativa
6.3.2.1 dimer 2 * 34000, SDS-PAGE Lotus japonicus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 Pantothenate synthetase
-
 Lotus japonicus
6.3.2.1 Pantothenate synthetase
-
 Oryza sativa
6.3.2.1 PS
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 0.62
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus
6.3.2.1 6.08
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.1 7.8
-
 in Tris-HCl Lotus japonicus
6.3.2.1 8
-
 in potassium phosphate Lotus japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.1 6 9 50% of maximal activity at pH 7.0, 15% of maximal activity at pH 6.0 and pH 9.0 respectively Lotus japonicus
6.3.2.1 7.1 9 in Tris-HCl, no activity at pH 7.0, 75% of maximal activity at pH 9.0 Lotus japonicus