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Literature summary extracted from

  • Tamura, M.; Ajayi, T.; Allmond, L.R.; Moriyama, K.; Wiener-Kronish, J.P.; Sawa, T.
    Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU (2004), Biochem. Biophys. Res. Commun., 316, 323-331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.5 glycerol 50% increase of lysophospholipase activity in the presence of 30% glycerol Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.5 expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 arachidonyl trifluoromethyl ketone 0.025 mM, approx. 50% inhibition Pseudomonas aeruginosa
3.1.1.5 bromoenol lactone 0.025 mM, approx. 30% inhibition Pseudomonas aeruginosa
3.1.1.5 trifluoromethyl ketone 0.025 mM, approx. 40% inhibition Pseudomonas aeruginosa
3.1.1.5 Triton X-100
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.5 additional information Ca2+ independent phospholipase A2 activity Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.5 2-lysophosphatidylcholine + H2O Pseudomonas aeruginosa
-
 glycerophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 recombinant ExoU Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1,2-palmitylphosphatidylcholine + H2O low activity after activating of ExoU with an unknown eukaryotic cell factor Pseudomonas aeruginosa 1-palmityl-lysophosphatidylcholine + palmitic acid
-
 ?
3.1.1.5 1-palmitoyl-lysophosphatidylcholine + H2O
-
 Pseudomonas aeruginosa glycerophosphocholine + palmitic acid
-
 ?
3.1.1.5 2-lysophosphatidylcholine + H2O
-
 Pseudomonas aeruginosa glycerophosphocholine + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 ExoU
-
 Pseudomonas aeruginosa
3.1.1.5 lysoPLA
-
 Pseudomonas aeruginosa