Literature summary extracted from
Ruf, A.; Muller, F.; D'Arcy, B.; Stihle, M.; Kusznir, E.; Handschin, C.; Morand, O.H.; Thoma, R.
The monotopic membrane protein human oxidosqualene cyclase is active as monomer (2004), Biochem. Biophys. Res. Commun., 315, 247-254.
Application
EC Number |
Application |
Comment |
Organism |
---|
5.4.99.7 |
medicine |
target for the development of new hypocjolesterolemic drugs |
Homo sapiens |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.99.7 |
expression in Pichia pastoris |
Homo sapiens |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.4.99.7 |
hanging-drop vapor-diffusion method |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.4.99.7 |
Ro 48-8071 |
IC50: 8.0 nM for the recombinant enzyme, IC50: 6.5 nM for the human liver enzyme |
Homo sapiens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
5.4.99.7 |
membrane |
monotopic, integral membrane protein |
Homo sapiens |
16020 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.4.99.7 |
80000 |
- |
1 * 80000, SDS-PAGE |
Homo sapiens |
5.4.99.7 |
86000 |
- |
equilibrium sedimentation |
Homo sapiens |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.4.99.7 |
(S)-2,3-epoxysqualene |
Homo sapiens |
formation of lanosterol the first sterol precursor of cholesterol in mammals |
lanosterol |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.99.7 |
Homo sapiens |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
5.4.99.7 |
additional information |
the protein is neither glycosylated nor modified post-translationally |
Homo sapiens |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.99.7 |
- |
Homo sapiens |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.4.99.7 |
-80°C, 10% glycerol, stable for at least 1 year |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.99.7 |
(S)-2,3-epoxysqualene |
- |
Homo sapiens |
lanosterol |
- |
? |
|
5.4.99.7 |
(S)-2,3-epoxysqualene |
formation of lanosterol the first sterol precursor of cholesterol in mammals |
Homo sapiens |
lanosterol |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.4.99.7 |
monomer |
1 * 80000, SDS-PAGE |
Homo sapiens |
IC50 Value
EC Number |
IC50 Value |
IC50 Value Maximum |
Comment |
Organism |
Inhibitor |
Structure |
---|
5.4.99.7 |
0.0000065 |
- |
IC50: 6.5 nM for the human liver enzyme |
Homo sapiens |
Ro 48-8071 |
|
5.4.99.7 |
0.000008 |
- |
IC50: 8.0 nM for the recombinant enzyme |
Homo sapiens |
Ro 48-8071 |
|