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Literature summary extracted from

  • Ruf, A.; Muller, F.; D'Arcy, B.; Stihle, M.; Kusznir, E.; Handschin, C.; Morand, O.H.; Thoma, R.
    The monotopic membrane protein human oxidosqualene cyclase is active as monomer (2004), Biochem. Biophys. Res. Commun., 315, 247-254.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.4.99.7 medicine target for the development of new hypocjolesterolemic drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.7 expression in Pichia pastoris Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.7 hanging-drop vapor-diffusion method Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.7 Ro 48-8071 IC50: 8.0 nM for the recombinant enzyme, IC50: 6.5 nM for the human liver enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.7 membrane monotopic, integral membrane protein Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.7 80000
-
1 * 80000, SDS-PAGE Homo sapiens
5.4.99.7 86000
-
equilibrium sedimentation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.7 (S)-2,3-epoxysqualene Homo sapiens formation of lanosterol the first sterol precursor of cholesterol in mammals lanosterol
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.4.99.7 additional information the protein is neither glycosylated nor modified post-translationally Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.7
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
5.4.99.7 -80°C, 10% glycerol, stable for at least 1 year Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (S)-2,3-epoxysqualene
-
Homo sapiens lanosterol
-
?
5.4.99.7 (S)-2,3-epoxysqualene formation of lanosterol the first sterol precursor of cholesterol in mammals Homo sapiens lanosterol
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.7 monomer 1 * 80000, SDS-PAGE Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.4.99.7 0.0000065
-
IC50: 6.5 nM for the human liver enzyme Homo sapiens Ro 48-8071
5.4.99.7 0.000008
-
IC50: 8.0 nM for the recombinant enzyme Homo sapiens Ro 48-8071