Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khan, F.; Jala, V.R.; Rao, N.A.; Savithri, H.S.
    Characterization of recombinant diaminopropionate ammonia-lyase from Escherichia coli and Salmonella typhimurium (2003), Biochem. Biophys. Res. Commun., 306, 1083-1088.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.15 0.17
-
 DL-2,3-diaminopropanoate pH 7.5, 37°C Escherichia coli
4.3.1.15 0.285
-
 D-serine pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 0.33
-
 DL-2,3-diaminopropanoate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 0.72
-
 D-serine pH 7.5, 37°C Escherichia coli
4.3.1.15 6.3
-
 L-serine pH 7.5, 37°C Escherichia coli
4.3.1.15 7.33
-
 L-serine pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.1.15 soluble
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
4.3.1.15 soluble
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.15 43000
-
 2 * 43000, SDS-PAGE Escherichia coli
4.3.1.15 44000
-
 x * 44000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 84000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.15 Escherichia coli
-
 recombinant enzyme
-
4.3.1.15 Salmonella enterica subsp. enterica serovar Typhimurium
-
 recombinant enzyme, expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.15 recombinant enzyme Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.3.1.15 about 60% reconstitution of apoenzyme by addition of pyridoxal 5'-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.15 0.21
-
 pH 7.5, 37°C, apoenzyme Escherichia coli
4.3.1.15 0.27
-
 pH 7.5, 37°C, apoenzyme Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 90.3
-
 pH 7.5, 37°C, holoenzyme Escherichia coli
4.3.1.15 191
-
 pH 7.5, 37°C, holoenzyme Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.15 D-serine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.3.1.15 D-serine + H2O
-
 Escherichia coli pyruvate + NH3
-
 ?
4.3.1.15 DL-2,3-diaminopropanoate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.3.1.15 DL-2,3-diaminopropanoate + H2O
-
 Escherichia coli pyruvate + NH3
-
 ?
4.3.1.15 L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.3.1.15 L-serine
-
 Escherichia coli pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.15 ? x * 44000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 dimer 2 * 43000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.15 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 pyridoxal 5'-phosphate
-
 Escherichia coli