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Literature summary extracted from

  • Yokoigawa, K.; Hirasawa, R.; Ueno, H.; Okubo, Y.; Umesako, S.; Soda, K.
    Gene cloning and characterization of alanine racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei (2001), Biochem. Biophys. Res. Commun., 288, 676-684.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 expression in Escherichia coli JM109 Shigella dysenteriae
5.1.1.1 expression in Escherichia coli JM109 Shigella boydii
5.1.1.1 expression in Escherichia coli JM109 Shigella flexneri
5.1.1.1 expression in Escherichia coli JM109 Shigella sonnei

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella boydii
5.1.1.1 Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella dysenteriae
5.1.1.1 Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella flexneri
5.1.1.1 Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella sonnei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 10
-
 D-Ala pH 8.3, 30°C Shigella dysenteriae
5.1.1.1 20
-
 L-Ala pH 8.3, 30°C Shigella dysenteriae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 43000
-
 1 * 43000, SDS-PAGE Shigella dysenteriae
5.1.1.1 43000
-
 1 * 43000, SDS-PAGE Shigella boydii
5.1.1.1 43000
-
 1 * 43000, SDS-PAGE Shigella flexneri
5.1.1.1 43000
-
 1 * 43000, SDS-PAGE Shigella sonnei
5.1.1.1 46000
-
 gel filtration Shigella dysenteriae
5.1.1.1 46000
-
 gel filtration Shigella boydii
5.1.1.1 46000
-
 gel filtration Shigella flexneri
5.1.1.1 46000
-
 gel filtration Shigella sonnei

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Shigella boydii P0A6B6
-
-
5.1.1.1 Shigella dysenteriae Q932V0
-
-
5.1.1.1 Shigella flexneri P0A6B5
-
-
5.1.1.1 Shigella sonnei Q93HP9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Shigella dysenteriae
5.1.1.1
-
 Shigella boydii
5.1.1.1
-
 Shigella flexneri
5.1.1.1
-
 Shigella sonnei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 209
-
 L-Ala, 30°C Shigella dysenteriae

Storage Stability

EC Number Storage Stability Organism
5.1.1.1 -80°C, over 60 days Shigella dysenteriae
5.1.1.1 -80°C, over 60 days Shigella boydii
5.1.1.1 -80°C, over 60 days Shigella flexneri
5.1.1.1 -80°C, over 60 days Shigella sonnei
5.1.1.1 4°C, 3 weeks Shigella dysenteriae
5.1.1.1 4°C, 3 weeks Shigella boydii
5.1.1.1 4°C, 3 weeks Shigella flexneri
5.1.1.1 4°C, 3 weeks Shigella sonnei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala
-
 Shigella dysenteriae D-Ala
-
 r
5.1.1.1 L-Ala
-
 Shigella boydii D-Ala
-
 r
5.1.1.1 L-Ala
-
 Shigella flexneri D-Ala
-
 r
5.1.1.1 L-Ala
-
 Shigella sonnei D-Ala
-
 r
5.1.1.1 L-Ser 2% of the activity with L-Ala Shigella dysenteriae D-Ser
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.1 monomer 1 * 43000, SDS-PAGE Shigella dysenteriae
5.1.1.1 monomer 1 * 43000, SDS-PAGE Shigella boydii
5.1.1.1 monomer 1 * 43000, SDS-PAGE Shigella flexneri
5.1.1.1 monomer 1 * 43000, SDS-PAGE Shigella sonnei
5.1.1.1 More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella dysenteriae
5.1.1.1 More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella boydii
5.1.1.1 More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella flexneri
5.1.1.1 More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella sonnei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 50
-
 in presence or absence of pyridoxal 5'-phosphate Shigella dysenteriae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.1 40
-
 1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella dysenteriae
5.1.1.1 40
-
 1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella boydii
5.1.1.1 40
-
 1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella flexneri
5.1.1.1 40
-
 1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella sonnei
5.1.1.1 50
-
 the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella dysenteriae
5.1.1.1 50
-
 the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella boydii
5.1.1.1 50
-
 the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella flexneri
5.1.1.1 50
-
 the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella sonnei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 8 10
-
 Shigella dysenteriae

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate cofactor Shigella dysenteriae
5.1.1.1 pyridoxal 5'-phosphate cofactor Shigella boydii
5.1.1.1 pyridoxal 5'-phosphate cofactor Shigella flexneri
5.1.1.1 pyridoxal 5'-phosphate cofactor Shigella sonnei