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Literature summary extracted from

  • Tang, X.F.; Ezaki, S.; Atomi, H.; Imanaka, T.
    Anthranilate synthase without an LLES motif from a hyperthermophilic archaeon is inhibited by tryptophan (2001), Biochem. Biophys. Res. Commun., 281, 858-865.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-tryptophan
-
Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.0034
-
chorismate 75°C, glutamine-dependent activity Thermococcus kodakarensis
4.1.3.27 0.0138
-
chorismate 75°C, ammonia-dependent activity Thermococcus kodakarensis
4.1.3.27 0.088
-
L-glutamine 75°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+ required, Km-value: 0.02 mM Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 21100
-
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence Thermococcus kodakarensis
4.1.3.27 23000
-
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence Thermococcus kodakarensis
4.1.3.27 48500
-
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence Thermococcus kodakarensis
4.1.3.27 57000
-
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence Thermococcus kodakarensis
4.1.3.27 60000
-
gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Thermococcus kodakarensis Q9YGB3 independent expression of subunits in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-glutamine
-
Thermococcus kodakarensis anthranilate + pyruvate + L-glutamate
-
?
4.1.3.27 additional information no substrate: asparagine Thermococcus kodakarensis ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 heterodimer 1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 85
-
-
Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 10
-
-
Thermococcus kodakarensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.27 0.005
-
L-tryptophan 75°C, ammonia-dependent activity, competitive to chorismate Thermococcus kodakarensis
4.1.3.27 0.074
-
L-tryptophan 75°C, glutamine-dependent activity, competitive to chorismate Thermococcus kodakarensis