EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.27 | L-tryptophan | - |
Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.27 | 0.0034 | - |
chorismate | 75°C, glutamine-dependent activity | Thermococcus kodakarensis | |
4.1.3.27 | 0.0138 | - |
chorismate | 75°C, ammonia-dependent activity | Thermococcus kodakarensis | |
4.1.3.27 | 0.088 | - |
L-glutamine | 75°C | Thermococcus kodakarensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.27 | Mg2+ | required, Km-value: 0.02 mM | Thermococcus kodakarensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 21100 | - |
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence | Thermococcus kodakarensis |
4.1.3.27 | 23000 | - |
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence | Thermococcus kodakarensis |
4.1.3.27 | 48500 | - |
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence | Thermococcus kodakarensis |
4.1.3.27 | 57000 | - |
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence | Thermococcus kodakarensis |
4.1.3.27 | 60000 | - |
gel filtration | Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.27 | Thermococcus kodakarensis | Q9YGB3 | independent expression of subunits in Escherichia coli | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.27 | chorismate + L-glutamine | - |
Thermococcus kodakarensis | anthranilate + pyruvate + L-glutamate | - |
? | |
4.1.3.27 | additional information | no substrate: asparagine | Thermococcus kodakarensis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.27 | heterodimer | 1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence | Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 85 | - |
- |
Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 10 | - |
- |
Thermococcus kodakarensis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.27 | 0.005 | - |
L-tryptophan | 75°C, ammonia-dependent activity, competitive to chorismate | Thermococcus kodakarensis | |
4.1.3.27 | 0.074 | - |
L-tryptophan | 75°C, glutamine-dependent activity, competitive to chorismate | Thermococcus kodakarensis |