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Literature summary extracted from

  • Dunn, M.F.; Araiza, G.; Finan, T.M.
    Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021 (2001), Arch. Microbiol., 176, 355-363.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetyl-CoA activates, half-maximal activity at 0.23 mM Sinorhizobium meliloti
6.4.1.1 propionyl-CoA activates less efficiently than acetyl-CoA Sinorhizobium meliloti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.1
-
 Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 2-oxoglutarate less than 10% inhibition at 5 mM Sinorhizobium meliloti
6.4.1.1 ADP 27% inhibition at 5 mM Sinorhizobium meliloti
6.4.1.1 L-aspartate 6.4% inhibition at 10 mM Sinorhizobium meliloti
6.4.1.1 L-glutamate less than 10% inhibition at 5 mM Sinorhizobium meliloti
6.4.1.1 NaCl slight inhibition at 20 mM Sinorhizobium meliloti
6.4.1.1 oxaloacetate inhibition below 1 mM Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.26
-
 pyruvate pH 8 Sinorhizobium meliloti
6.4.1.1 0.66
-
 ATP pH 8 Sinorhizobium meliloti
6.4.1.1 5.6
-
 HCO3- pH 8 Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 KCl 1.6fold stimulation at 20 mM Sinorhizobium meliloti
6.4.1.1 MgCl2 7.5 mM Sinorhizobium meliloti
6.4.1.1 MnCl2 7.5 mM, can partially replace MgCl2 Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 120000
-
 4 * 120000, SDS-PAGE Sinorhizobium meliloti
6.4.1.1 500000
-
 gel filtration Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3- Sinorhizobium meliloti
-
 ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- Sinorhizobium meliloti Rm1021
-
 ADP + phosphate + oxaloacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Sinorhizobium meliloti
-
 Rm1021
-
6.4.1.1 Sinorhizobium meliloti Rm1021
-
 Rm1021
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Sinorhizobium meliloti ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3-
-
 Sinorhizobium meliloti Rm1021 ADP + phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 tetramer 4 * 120000, SDS-PAGE Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin activity is dependent on biotin availability Sinorhizobium meliloti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.4.1.1 0.22
-
 oxaloacetate pH 8 Sinorhizobium meliloti