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Literature summary extracted from

  • Mukhopadhyay, B.; Patel, V.J.; Wolfe, R.S.
    A stable archaeal pyruvate carboxylase from the hyperthermophile Methanococcus jannaschii (2000), Arch. Microbiol., 174, 406-414.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 ADP
-
 Methanocaldococcus jannaschii
6.4.1.1 ATP
-
 Methanocaldococcus jannaschii
6.4.1.1 Avidin 100 molar excess amount completely inhibits after 5 min Methanocaldococcus jannaschii
6.4.1.1 KCl activates at lower concentrations, inhibits at higher concentrations Methanocaldococcus jannaschii
6.4.1.1 Mg2+ when Mg2+ concentration in the assay exceeds that of ATP Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.22
-
 HCO3- 80°C, pH 8.5 Methanocaldococcus jannaschii
6.4.1.1 0.374
-
 ATP 80°C, pH 8.5 Methanocaldococcus jannaschii
6.4.1.1 0.53
-
 pyruvate 80°C, pH 8.5 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 Co2+ can poorly replace Mg2+ Methanocaldococcus jannaschii
6.4.1.1 KCl optimal concentration: 0.175 mM, activates at lower concentrations, inhibits at higher concentrations Methanocaldococcus jannaschii
6.4.1.1 Mg2+ required, inhibits at higher concentrations Methanocaldococcus jannaschii
6.4.1.1 Mn2+ can poorly replace Mg2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 55000
-
 alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii
6.4.1.1 65000
-
 alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii
6.4.1.1 480000
-
 gel filtration, Stokes' radius Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3- Methanocaldococcus jannaschii
-
 ADP + phosphate + oxaloacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
6.4.1.1 -20°C, stable, purified enzyme, 75% activity after 5 days Methanocaldococcus jannaschii
6.4.1.1 -80°C, stable, purified enzyme, 75% activity after 5 days Methanocaldococcus jannaschii
6.4.1.1 4°C, stable, purified enzyme, 83-85% activity after 36 h Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Methanocaldococcus jannaschii ADP + phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 octamer alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.1 80 90
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.1 80
-
 rapid inactivation, t1/2: 1 h in 100 mM Tris/HCl, pH 7, sulfate salts provide partial protection Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.1 8.5
-
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin
-
 Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.4.1.1 0.89
-
 ADP 80°C, pH 8.5 Methanocaldococcus jannaschii
6.4.1.1 5.34
-
 ATP 80°C, pH 8.5 Methanocaldococcus jannaschii