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Literature summary extracted from
- Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase (2003), Arch. Biochem. Biophys., 415, 14-23.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.1.3 | Ca2+ | 10% activation at 0.1 mM | Rhodococcus erythropolis |  |
| 3.13.1.3 | additional information | no activation by metal ions | Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.1.3 | Co2+ | 18% inhibition at 0.1 mM | Rhodococcus erythropolis | |
| 3.13.1.3 | Cu2+ | 95% inhibition at 0.1 mM | Rhodococcus erythropolis | |
| 3.13.1.3 | DTNB | complete inhibition at 0.01 mM after 10 min | Rhodococcus erythropolis | |
| 3.13.1.3 | Fe2+ | 11% inhibition at 0.1 mM | Rhodococcus erythropolis | |
| 3.13.1.3 | additional information | no product inhibition, no inhibition by Mn2+, Mg2+, and Ca2 | Rhodococcus erythropolis | |
| 3.13.1.3 | N-bromosuccimide | complete inhibition at 0.01 mM after 10 min | Rhodococcus erythropolis | |
| 3.13.1.3 | phenylglyoxal hydrate | 19% inhibition at 0.01 mM after 10 min | Rhodococcus erythropolis | |
| 3.13.1.3 | pyridoxal-5-phosphate | 38% inhibition at 0.01 mM after 10 min | Rhodococcus erythropolis | |
| 3.13.1.3 | Tetranitromethane | complete inhibition at 0.01 mM after 10 min | Rhodococcus erythropolis | |
| 3.13.1.3 | Zn2+ | 50% inhibition at 0.1 mM | Rhodococcus erythropolis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.1.3 | 0.0009 | - |
2-(2'-hydroxyphenyl)benzenesulfinate | pH 7.4, 35°C | Rhodococcus erythropolis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 40000 | - |
x * 40000, SDS-PAGE | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | Rhodococcus erythropolis | final step of the desulfurozation of dibenzothiophene | 2-hydroxybiphenyl + sulfite | - |
? | |
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | Rhodococcus erythropolis IGTS8 / ATCC 53968 | final step of the desulfurozation of dibenzothiophene | 2-hydroxybiphenyl + sulfite | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.1.3 | Rhodococcus erythropolis | - |
- |
- |
| 3.13.1.3 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.1.3 | 1600fold | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 0.00046 | - |
purified enzyme | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | specific for | Rhodococcus erythropolis | 2-hydroxybiphenyl + sulfite | - |
? | |
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | final step of the desulfurozation of dibenzothiophene | Rhodococcus erythropolis | 2-hydroxybiphenyl + sulfite | - |
? | |
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | specific for | Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2-hydroxybiphenyl + sulfite | - |
? | |
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate + H2O | final step of the desulfurozation of dibenzothiophene | Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2-hydroxybiphenyl + sulfite | - |
? | |
| 3.13.1.3 | additional information | no activity with xanthene, 2-hydroxydiphenylmetane, 2-methoxybiphenyl, phenylhydroquinone, 2-biphenyl carboxylic acid, 2-amino-4-phenylphenol | Rhodococcus erythropolis | ? | - |
? | |
| 3.13.1.3 | additional information | no activity with xanthene, 2-hydroxydiphenylmetane, 2-methoxybiphenyl, phenylhydroquinone, 2-biphenyl carboxylic acid, 2-amino-4-phenylphenol | Rhodococcus erythropolis IGTS8 / ATCC 53968 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.1.3 | ? | x * 40000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.1.3 | 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 35 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 25 | 50 | rapid loss of activity at temperatures higher or lower than the optimum | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 7 | - |
- |
Rhodococcus erythropolis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.3 | 6 | 8.4 | activity decreases sigificantly below pH 6.0 and above pH 8.4 | Rhodococcus erythropolis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.13.1.3 | Rhodococcus erythropolis | isoelectric focusing | 5.9 | 5.6 |
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