EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.2.13 | medicine | mutations in aldolase B gene cause hereditary fructose intolerance, most common intolerance allele encodes a A149P substitution | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.13 | enzyme from patients with fructose intolerance | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.13 | 0.0017 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 0.0024 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 0.0028 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.0085 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.24 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 0.88 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 4.2 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 16.4 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.13 | 57000 | - |
sucrose density gradient centrifugation, 30°C | Homo sapiens |
4.1.2.13 | 71000 | - |
sucrose density gradient centrifugation, 4°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.13 | D-fructose 1,6-bisphosphate | Homo sapiens | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.13 | Homo sapiens | - |
patients with fructose intolerance | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.13 | enzyme from patients with fructose intolerance | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.13 | D-fructose 1,6-bisphosphate | - |
Homo sapiens | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.13 | D-Fructose 1-phosphate | - |
Homo sapiens | Glycerone phosphate + D-glyceraldehyde | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.13 | dimer | gel filtration, enzyme from patients with fructose intolerance | Homo sapiens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.13 | 15 | - |
gradual loss of activity above, enzyme from patients with fructose intolerance | Homo sapiens |
4.1.2.13 | 40 | - |
wild-type enzyme, activity is stable up to 40°C, declines sharply above 40°C | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.13 | 10 | - |
enzyme from patients with fructose intolerance shows increased temperature sensitivity, activity at 10°C is 6fold lower than that of the wild-type, and activity decreases substantially at higher temperatures | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.13 | 0.008 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.011 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.019 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.029 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, enzyme from patients with intolerance | Homo sapiens | |
4.1.2.13 | 0.12 | - |
D-Fructose 1-phosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 0.19 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 10°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 2 | - |
D-Fructose 1-phosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens | |
4.1.2.13 | 2.1 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 30°C, wild-type enzyme | Homo sapiens |