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Literature summary extracted from

  • Rokov-Plavec, J.; Lesjak, S.; Landeka, I.; Mijakovic, I.; Weygand-Durasevic, I.
    Maize seryl-tRNA synthetase: specificity of substrate recognition by the organellar enzyme (2002), Arch. Biochem. Biophys., 397, 40-50.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 expression of the mitochondrial isozyme in an enzyme-deficient Saccharomyces cerevisiae strain, caused by gene disruption, leads to functional complementation, overexpression of the mitochondrial isozyme in Escherichia coli BL21(DE3) Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 0.00025
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.0004
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.0005
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.00067
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.04
-
 L-serine mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C Zea mays
6.1.1.11 0.45
-
 L-serine mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.5, 30°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.11 cytoplasm cytoplasmic isozyme Zea mays 5737
-
6.1.1.11 mitochondrion mitochondrial isozyme SerZMm Zea mays 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Zea mays
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information Zea mays phylogenetic comparison ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Zea mays
-
 2 isozymes, one mitochondrial and another cytoplasmic
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 recombinant mitochondrial isozyme from overexpression in Escherichia coli BL21(DE3), to homogeneity Zea mays

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.11 ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer subtrate recognition mechanism Zea mays
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer enzyme utilizes tRNA substrates from Zea mays, Saccharomyces cerevisiae, and Escherichia coli, recombinant mitochondrial isozyme Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer tRNASer from Escherichia coli Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer chloroplastic tRNASer from maize Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer mitochondrial tRNASer from maize Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer mitochondrial tRNASer from yeast subtype 2 Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information no activity with tRNASer from Arabidopsis thaliana, the enzyme also performs the ATP-diphosphate exchange reaction Zea mays ?
-
 ?
6.1.1.11 additional information phylogenetic comparison Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 62 kDa RNA-binding protein
-
 Zea mays
6.1.1.11 Serine transfer RNA synthetase
-
 Zea mays
6.1.1.11 Serine translase
-
 Zea mays
6.1.1.11 Serine--tRNA ligase
-
 Zea mays
6.1.1.11 SerRS
-
 Zea mays
6.1.1.11 SerRSmt
-
 Zea mays
6.1.1.11 SERSEC
-
 Zea mays
6.1.1.11 Seryl-transfer ribonucleate synthetase
-
 Zea mays
6.1.1.11 Seryl-transfer ribonucleic acid synthetase
-
 Zea mays
6.1.1.11 Seryl-transfer RNA synthetase
-
 Zea mays
6.1.1.11 Seryl-tRNA synthetase
-
 Zea mays
6.1.1.11 Synthetase, seryl-transfer ribonucleate
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 30
-
 assay at Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.11 0.2
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.5
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 0.9
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 1.2
-
 L-serine mitochondrial isozyme, aminoacylation reaction, pH 7.5, 30°C Zea mays
6.1.1.11 1.2
-
 tRNASer mitochondrial isozyme, pH 7.5, 30°C Zea mays
6.1.1.11 6.9
-
 L-serine mitochondrial isozyme, ATP-diphosphate exchange reaction, pH 7.2, 30°C Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Zea mays