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Literature summary extracted from

  • Jung, C.H.; Hartman, F.C.; Lu, T.Y.S.; Larimer, F.W.
    D-Ribose-5-phosphate isomerase from spinach: heterologous overexpression, purification, characterization, and site-directed mutagenesis of the recombinant enzyme (2000), Arch. Biochem. Biophys., 373, 409-417.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 overexpression in Escherichia coli Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.6 D87A turnover number with D-ribose-5-phosphate is 0.0012% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
5.3.1.6 D90A turnover number with D-ribose-5-phosphate is 0.38% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
5.3.1.6 E91A turnover number with D-ribose-5-phosphate is 27.5% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
5.3.1.6 K100A turnover number with D-ribose-5-phosphate is 0.074% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.6 arabinose 5-phosphate
-
 Spinacia oleracea
5.3.1.6 D-arabinose 5-phosphate effective Spinacia oleracea
5.3.1.6 phosphate competitive Spinacia oleracea
5.3.1.6 Xylulose 5-phosphate
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.6 0.52
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D90A Spinacia oleracea
5.3.1.6 0.56
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D87A Spinacia oleracea
5.3.1.6 0.63
-
 D-ribose-5-phosphate pH 8.0, 25°C, wild-type enzyme Spinacia oleracea
5.3.1.6 1.17
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme E91A Spinacia oleracea
5.3.1.6 4.41
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 25000
-
 2 * 25000, SDS-PAGE Spinacia oleracea
5.3.1.6 25066
-
 2 * 25066, calculation from nucleotide sequence Spinacia oleracea
5.3.1.6 49000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 recombinant enzyme Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.6 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate
-
 Spinacia oleracea D-ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 dimer 2 * 25000, SDS-PAGE Spinacia oleracea
5.3.1.6 dimer 2 * 25066, calculation from nucleotide sequence Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 RPI
-
 Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.6 0.0421
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D87A Spinacia oleracea
5.3.1.6 2.5 4 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea
5.3.1.6 2.54
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea
5.3.1.6 13.2
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D90A Spinacia oleracea
5.3.1.6 946
-
 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme E91A Spinacia oleracea
5.3.1.6 3440
-
 D-ribose-5-phosphate pH 8.0, 25°C, wild-type enzyme Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.6 0.7
-
 arabinose 5-phosphate pH 8.0, 25°C Spinacia oleracea
5.3.1.6 4
-
 Xylulose 5-phosphate pH 8.0, 25°C Spinacia oleracea
5.3.1.6 7.9
-
 phosphate pH 8.0, 25°C Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.6 Spinacia oleracea isoelectric focusing
-
 5.1