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Literature summary extracted from

  • Tye, A.J.; Siu, F.K.Y.; Leung, T.Y.C.; Lim, B.L.
    Molecular cloning and the biochemical characterization of two novel phytases from B. subtilis 168 and B. licheniformis (2002), Appl. Microbiol. Biotechnol., 59, 190-197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 overexpression in Bacillus subtilis using a PHI105MU331 prophage vector system Bacillus licheniformis
3.1.3.26 overexpression in Bacillus subtilis using a PHI105MU331 prophage vector system Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 44000
-
 x * 44000, SDS-PAGE Bacillus subtilis
3.1.3.8 47000
-
 x * 47000, SDS-PAGE Bacillus licheniformis
3.1.3.26 44000
-
 x * 44000, SDS-PAGE Bacillus subtilis
3.1.3.26 47000
-
 x * 47000, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Bacillus licheniformis Q8KTX7 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Bacillus subtilis
-
-
-
3.1.3.26 Bacillus licheniformis Q8KTX7 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Bacillus licheniformis
3.1.3.26
-
 Bacillus licheniformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 culture medium recombinant enzyme in culture medium of Bacillus subtilis Bacillus licheniformis
-
3.1.3.26 culture medium recombinant enzyme in culture medium of Bacillus subtilis Bacillus licheniformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Bacillus subtilis ? + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Bacillus licheniformis ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Bacillus subtilis ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Bacillus licheniformis ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 47000, SDS-PAGE Bacillus licheniformis
3.1.3.8 ? x * 44000, SDS-PAGE Bacillus subtilis
3.1.3.26 ? x * 47000, SDS-PAGE Bacillus licheniformis
3.1.3.26 ? x * 44000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
 Bacillus subtilis
3.1.3.8 65
-
-
 Bacillus licheniformis
3.1.3.26 55
-
-
 Bacillus subtilis
3.1.3.26 65
-
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 37 65 37°C: about 40% of maximal activity, 65°C: about 40% of maximal activity Bacillus subtilis
3.1.3.8 45 70 45°C: 45% of maximal activity, 70°C: about 55% of maximal activity Bacillus licheniformis
3.1.3.26 37 65 37°C: about 40% of maximal activity, 65°C: about 40% of maximal activity Bacillus subtilis
3.1.3.26 45 70 45°C: 45% of maximal activity, 70°C: about 55% of maximal activity Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 70
-
 10 min, about 15% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.8 70
-
 10 min, about 70% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.8 75
-
 10 min, about 35% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.8 75
-
 10 min, about 65% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.8 80
-
 10 min, about 35% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.8 80
-
 10 min, about 60% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.8 85
-
 10 min, about 30% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.8 85
-
 10 min, about 45% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.8 90
-
 10 min, about 30% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.8 90
-
 10 min, about 65% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.8 95
-
 about 40% loss of activity after 15 min, about 60% loss of activity after 30 min, in presence of 5 mM ca2+ Bacillus licheniformis
3.1.3.8 95
-
 about 55% loss of activity after 15 min, about 75% loss of activity after 30 min, with 5 mM Ca2+ Bacillus subtilis
3.1.3.26 70
-
 10 min, about 15% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.26 70
-
 10 min, about 70% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.26 75
-
 10 min, about 35% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.26 75
-
 10 min, about 65% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.26 80
-
 10 min, about 35% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.26 80
-
 10 min, about 60% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.26 85
-
 10 min, about 30% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.26 85
-
 10 min, about 45% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.26 90
-
 10 min, about 30% loss of activity, in presence of 1 mM Ca2+ Bacillus licheniformis
3.1.3.26 90
-
 10 min, about 65% loss of activity, with 1 mM Ca2+ Bacillus subtilis
3.1.3.26 95
-
 about 40% loss of activity after 15 min, about 60% loss of activity after 30 min, in presence of 5 mM ca2+ Bacillus licheniformis
3.1.3.26 95
-
 about 55% loss of activity after 15 min, about 75% loss of activity after 30 min, with 5 mM Ca2+ Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6
-
 broad Bacillus subtilis
3.1.3.8 6
-
 broad Bacillus licheniformis
3.1.3.26 6
-
 broad Bacillus subtilis
3.1.3.26 6
-
 broad Bacillus licheniformis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Bacillus subtilis two-dimensional electrophoresis
-
 5
3.1.3.8 Bacillus licheniformis two-dimensional electrophoresis
-
 5.1
3.1.3.26 Bacillus subtilis two-dimensional electrophoresis
-
 5
3.1.3.26 Bacillus licheniformis two-dimensional electrophoresis
-
 5.1