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Literature summary extracted from

  • Rehm, B.H.; Steinbuchel, A.
    Heterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia californica mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia coli (2001), Appl. Microbiol. Biotechnol., 55, 205-209.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 functional expression in Escherichia coli, diverse recombinant mutant strains Umbellularia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 additional information Umbellularia californica the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview ?
-
 ?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O Umbellularia californica
-
 oleic acid + acyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Umbellularia californica
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.14 15
-
 recombinant enzyme in Escherichia coli, substrate lauroyl-CoA Umbellularia californica
3.1.2.14 1500
-
 recombinant enzyme in Escherichia coli, substrate lauroyl-[acyl-carrier-protein] Umbellularia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 lauroyl-CoA + H2O
-
 Umbellularia californica lauric acid + CoA
-
 ?
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica lauric acid + acyl-carrier protein
-
 ?
3.1.2.14 additional information the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview Umbellularia californica ?
-
 ?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica oleic acid + acyl-carrier protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 12:0-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 12:0-acyl-carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 14:0-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 14:0-acyl-carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 16:0-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 16:0-acyl-carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 18:0-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 18:0-acyl-carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 acyl-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 acyl-ACP-hydrolase
-
 Umbellularia californica
3.1.2.14 acyl-acyl carrier protein hydrolase
-
 Umbellularia californica
3.1.2.14 acyl-acyl carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 acyl-[acyl-carrier-protein] hydrolase
-
 Umbellularia californica
3.1.2.14 AH
-
 Umbellularia californica
3.1.2.14 BTE
-
 Umbellularia californica
3.1.2.14 C12:0 ACP TE
-
 Umbellularia californica
3.1.2.14 C18:1-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 Deubiquitinating enzyme 14
-
 Umbellularia californica
3.1.2.14 hydrolase, oleoyl-[acyl carrier protein]
-
 Umbellularia californica
3.1.2.14 oleoyl-ACP thioesterase
-
 Umbellularia californica
3.1.2.14 oleoyl-acyl carrier protein thioesterase
-
 Umbellularia californica
3.1.2.14 OTE
-
 Umbellularia californica
3.1.2.14 PATE
-
 Umbellularia californica
3.1.2.14 STE
-
 Umbellularia californica
3.1.2.14 Ubiquitin thiolesterase 14
-
 Umbellularia californica
3.1.2.14 Ubiquitin-specific processing protease 14
-
 Umbellularia californica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.14 30
-
 assay at Umbellularia californica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.14 8
-
 assay at Umbellularia californica