EC Number | Application | Comment | Organism |
---|---|---|---|
5.3.1.4 | nutrition | the enzyme may be a good model for analysis of metal-mediated thermostabilization and for industrial application in the production of D-tagatose as a novel sweetener | Thermotoga maritima |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.4 | overexpression in Escherichia coli as a fusion protein containing a C-terminal hexahistidine sequence | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.4 | 31 | - |
L-arabinose | pH 7.5, 90°C | Thermotoga maritima | |
5.3.1.4 | 60 | - |
D-galactose | pH 7.5, 90°C | Thermotoga maritima |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.4 | Ca2+ | poor activator | Thermotoga maritima | |
5.3.1.4 | Co2+ | Mn2+ or Co2+ required, about 1 mM Co2+ restores activity of dialyzed enzyme | Thermotoga maritima | |
5.3.1.4 | Fe2+ | poor activator | Thermotoga maritima | |
5.3.1.4 | Mg2+ | poor activator | Thermotoga maritima | |
5.3.1.4 | Mn2+ | Mn2+ or Co2+ required, about 5 mM Mn2+ restores activity of dialyzed enzyme | Thermotoga maritima | |
5.3.1.4 | Ni2+ | poor activator | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 56658 | - |
4 * 56658, calculation from nucleotide sequence | Thermotoga maritima |
5.3.1.4 | 57000 | - |
4 * 57000, SDS-PAGE | Thermotoga maritima |
5.3.1.4 | 230000 | - |
gel filtration | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.4 | Thermotoga maritima | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.4 | - |
Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | D-galactose | 56% conversion after 6 h at 80°C | Thermotoga maritima | D-tagatose | - |
? | |
5.3.1.4 | L-arabinose | 70% conversion to L-ribulose in 6 h at 80°C | Thermotoga maritima | L-ribulose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.4 | tetramer | 4 * 57000, SDS-PAGE | Thermotoga maritima |
5.3.1.4 | tetramer | 4 * 56658, calculation from nucleotide sequence | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.4 | AI | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 85 | - |
in absence of metal ions | Thermotoga maritima |
5.3.1.4 | 90 | - |
in presence of metal ions | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | additional information | - |
more thermostable in presence of Mn2+ and/or Co2+ than in absence | Thermotoga maritima |
5.3.1.4 | 80 | - |
240 min, incubation in presence of Mn2+ or Co2+ causes little loss of activity, apoenzyme is very unstable in absence of metal ions | Thermotoga maritima |
5.3.1.4 | 90 | - |
half-life in presence of 5 mM Mn2+: 185 min | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 7.5 | - |
90°C, in presence of 1 mM Co2+ and 5 mM Mn2+ | Thermotoga maritima |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.3.1.4 | Thermotoga maritima | isoelectric focusing, pH-gradient: pH 3.0-10.0 | - |
5.7 |