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Literature summary extracted from

  • Muraki, T.; Taki, M.; Hasegawa, Y.; Iwaki, H.; Lau, P.C.
    Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7 (2003), Appl. Environ. Microbiol., 69, 1564-1572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.6 expression in Escherichia coli Pseudomonas fluorescens
4.1.1.45 nbaD gene overexpressed in Escherichia coli Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.6 H52A inactive enzyme Pseudomonas fluorescens
1.13.11.6 H93A 24.8% activity of the native enzyme Pseudomonas fluorescens
1.13.11.6 H96A inactive enzyme Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.6 1,10-phenanthroline Fe2+ chelator, 1 mM, complete inhibition Pseudomonas fluorescens
1.13.11.6 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide methiodide carboxyl-directed reagent, 1 mM, 26% inhibition Pseudomonas fluorescens
1.13.11.6 2,2'-dipyridyl Fe2+ chelator, 1 mM, complete inhibition Pseudomonas fluorescens
1.13.11.6 Cd2+ 0.1 mM, more than 99% inhibition Pseudomonas fluorescens
1.13.11.6 Co2+ 1 mM, 92% inhibition Pseudomonas fluorescens
1.13.11.6 Cu2+ 0.1 mM, complete inhibition Pseudomonas fluorescens
1.13.11.6 diethyl dicarbonate modifies histidine residues of catechol dioxygenases, 1 mM, 70% inhibition Pseudomonas fluorescens
1.13.11.6 Dithionitrobenzoic acid cysteine-directed reagent, 1 mM, complete inhibition Pseudomonas fluorescens
1.13.11.6 EDTA 1 mM, 99% inhibition Pseudomonas fluorescens
1.13.11.6 Fe3+ 0.1 mM, 82% inhibition Pseudomonas fluorescens
1.13.11.6 iodoacetate cysteine-directed reagent, 1 mM, 42% inhibition Pseudomonas fluorescens
1.13.11.6 Ni2+ 1 mM, 82% inhibition Pseudomonas fluorescens
1.13.11.6 Zn2+ 0.1 mM, 99% inhibition Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.6 Fe2+ needed for catalytic activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.6 21240
-
 predicted from cDNA sequence Pseudomonas fluorescens
1.13.11.6 23800
-
 SDS-PAGE Pseudomonas fluorescens
4.1.1.45 37140
-
 deduced from DNA sequence Pseudomonas fluorescens
4.1.1.45 38700
-
 recombinant protein, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2 Pseudomonas fluorescens
-
 2-amino-3-carboxymuconate semialdehyde
-
 ?
1.13.11.6 3-hydroxyanthranilate + O2 Pseudomonas fluorescens KU-7
-
 2-amino-3-carboxymuconate semialdehyde
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate Pseudomonas fluorescens 2-nitrobenzoate degradation pathway 2-aminomuconate-6-semialdehyde + CO2
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate Pseudomonas fluorescens KU-7 2-nitrobenzoate degradation pathway 2-aminomuconate-6-semialdehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Pseudomonas fluorescens Q83V26
-
-
1.13.11.6 Pseudomonas fluorescens KU-7 Q83V26
-
-
4.1.1.45 Pseudomonas fluorescens Q83V25
-
-
4.1.1.45 Pseudomonas fluorescens KU-7 Q83V25
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.6 7.9
-
 3-hydroxyanthranilate as substrate, pH 6.5, 30ºC Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2
-
 Pseudomonas fluorescens 2-amino-3-carboxymuconate semialdehyde
-
 ?
1.13.11.6 3-hydroxyanthranilate + O2
-
 Pseudomonas fluorescens KU-7 2-amino-3-carboxymuconate semialdehyde
-
 ?
1.13.11.6 additional information no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates Pseudomonas fluorescens ?
-
 ?
1.13.11.6 additional information no detectable activity with 3-amino-4-hydroxybenzoic acid, 4-aminoresorcinol, 2-amino-m-cresol, 4-amino-3-hydroxybenzoic acid, 3-aminosalicylic acid, 6-amino-m-cresol, 3-methylcatechol, 4-methylcatechol, 1,2,4-trihydroxybenzene, 2,3-dihydroxybenzoic acid, 4-amino-m-cresol, 5-aminosalicylic acid, gentisic acid, homogentisic acid, 2-amino-4-chlorophenol, 2-amino-p-cresol, catechol, 1,2,3-trihydroxybenzene, protocatechuic acid, hydroquinone as substrates Pseudomonas fluorescens KU-7 ?
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate
-
 Pseudomonas fluorescens 2-aminomuconate-6-semialdehyde + CO2
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate 2-nitrobenzoate degradation pathway Pseudomonas fluorescens 2-aminomuconate-6-semialdehyde + CO2
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate
-
 Pseudomonas fluorescens KU-7 2-aminomuconate-6-semialdehyde + CO2
-
 ?
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate 2-nitrobenzoate degradation pathway Pseudomonas fluorescens KU-7 2-aminomuconate-6-semialdehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.45 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase
-
 Pseudomonas fluorescens
4.1.1.45 NbaD enzyme
-
 Pseudomonas fluorescens