EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.43 | expression in Escherichia coli | Acetobacter pasteurianus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.43 | 0.34 | - |
cephalexin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 1 | - |
ampicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 1.1 | - |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 1.7 | - |
cefadroxil | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 2.6 | - |
amoxicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 7 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 13 | - |
D-phenylglycine amide | 30°C, pH 6.2 | Acetobacter pasteurianus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.43 | 70000 | - |
2 * 70000, SDS-PAGE | Acetobacter pasteurianus |
3.1.1.43 | 150000 | - |
dynamic light scattering | Acetobacter pasteurianus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.43 | an alpha-aminoacid ester + H2O | Acetobacter pasteurianus | - |
an alpha-amino acid + an alcohol | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.43 | Acetobacter pasteurianus | Q8VRK8 | ATCC 9325 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.43 | CM-Sepharose, hydrophobic interaction, gel filtration | Acetobacter pasteurianus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.43 | 667 | - |
Cephalexin synthesis | Acetobacter pasteurianus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.43 | amoxicillin + H2O | - |
Acetobacter pasteurianus | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
? | |
3.1.1.43 | ampicillin + H2O | - |
Acetobacter pasteurianus | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
r | |
3.1.1.43 | an alpha-aminoacid ester + H2O | - |
Acetobacter pasteurianus | an alpha-amino acid + an alcohol | - |
r | |
3.1.1.43 | cefadroxil + H2O | - |
Acetobacter pasteurianus | ? | - |
r | |
3.1.1.43 | cephalexin + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + 7-aminodesacetoxycephalosporanic acid | - |
r | |
3.1.1.43 | D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O | - |
Acetobacter pasteurianus | phenylglycine + p-nitrophenol | - |
r | |
3.1.1.43 | D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O | - |
Acetobacter pasteurianus | ? | - |
? | |
3.1.1.43 | D-phenylglycine amide + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + NH3 | - |
r | |
3.1.1.43 | D-phenylglycine methyl ester + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + methanol | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.43 | dimer | 2 * 70000, SDS-PAGE | Acetobacter pasteurianus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.43 | 0.4 | - |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 9.6 | - |
cefadroxil | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 10 | - |
amoxicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 43 | - |
D-phenylglycine amide | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 162 | - |
ampicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 347 | - |
cephalexin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 1035 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus | |
3.1.1.43 | 1040 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus |