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Literature summary extracted from

  • Lassen, S.F.; Breinholt, J.; Ostergaard, P.R.; Brugger, R.; Bischoff, A.; Wyss, M.; Fuglsang, C.C.
    Expression, gene cloning, and characterization of five novel phytases from four basidiomycete fungi: Peniophora lycii, Agrocybe pediades, a Ceriporia sp., and Trametes pubescens (2001), Appl. Environ. Microbiol., 67, 4701-4707.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.26 expression in Aspergillus oryzae Agrocybe pediades
3.1.3.26 expression in Aspergillus oryzae Ceriporia sp.
3.1.3.26 expression in Aspergillus oryzae Trametes pubescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 54000
-
 x * 54000, SDS-PAGE Ceriporia sp.
3.1.3.26 59000
-
 x * 59000, SDS-PAGE Agrocybe pediades
3.1.3.26 59000
-
 x * 59000, SDS-PAGE Ceriporia sp.
3.1.3.26 62000
-
 x * 62000, SDS-PAGE Trametes pubescens
3.1.3.26 72000
-
 x * 72000, SDS-PAGE Peniophora lycii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Agrocybe pediades Q96VT0
-
-
3.1.3.26 Ceriporia sp. Q96VK8
-
-
3.1.3.26 Ceriporia sp. Q96VK9
-
-
3.1.3.26 Peniophora lycii Q96VH9
-
-
3.1.3.26 Trametes pubescens Q96VF5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 glycoprotein 10 potential glycosylation sites Peniophora lycii
3.1.3.26 glycoprotein 6 potential glycosylation sites Agrocybe pediades
3.1.3.26 glycoprotein 7 potential glycosylation sites Ceriporia sp.
3.1.3.26 glycoprotein 8 potential glycosylation sites Ceriporia sp.
3.1.3.26 glycoprotein 9 potential glycosylation sites Trametes pubescens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 recombinant enzyme Agrocybe pediades
3.1.3.26 recombinant enzyme Ceriporia sp.
3.1.3.26 recombinant enzyme Trametes pubescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 400
-
-
 Agrocybe pediades
3.1.3.26 700
-
-
 Ceriporia sp.
3.1.3.26 1040
-
-
 Ceriporia sp.
3.1.3.26 1080
-
-
 Peniophora lycii
3.1.3.26 1210
-
-
 Trametes pubescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Agrocybe pediades ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Ceriporia sp. ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Trametes pubescens ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Peniophora lycii ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 ? x * 54000, SDS-PAGE Ceriporia sp.
3.1.3.26 ? x * 72000, SDS-PAGE Peniophora lycii
3.1.3.26 ? x * 62000, SDS-PAGE Trametes pubescens
3.1.3.26 ? x * 59000, SDS-PAGE Agrocybe pediades
3.1.3.26 ? x * 59000, SDS-PAGE Ceriporia sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 PhyA1
-
 Ceriporia sp.
3.1.3.26 PhyA2
-
 Ceriporia sp.
3.1.3.26 PhyA2
-
 Trametes pubescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 40 45
-
 Ceriporia sp.
3.1.3.26 40 45
-
 Trametes pubescens
3.1.3.26 50
-
-
 Agrocybe pediades
3.1.3.26 50 55
-
 Peniophora lycii
3.1.3.26 55 60
-
 Ceriporia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 80
-
 pH 5.5, 60 min, 38% loss of activity Peniophora lycii
3.1.3.26 80
-
 pH 5.5, 60 min, 62% loss of activity Ceriporia sp.
3.1.3.26 80
-
 pH 5.5, 60 min, 78% loss of activity Ceriporia sp.
3.1.3.26 80
-
 pH 5.5, 60 min, 85% loss of activity Trametes pubescens
3.1.3.26 80
-
 pH 5.5, 60 min,53% loss of activity Agrocybe pediades

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4 4.5
-
 Peniophora lycii
3.1.3.26 5 5.5
-
 Trametes pubescens
3.1.3.26 5 6
-
 Agrocybe pediades
3.1.3.26 5 6
-
 Ceriporia sp.
3.1.3.26 5.5 6
-
 Ceriporia sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.26 Trametes pubescens isoelectric focusing
-
 3.58
3.1.3.26 Peniophora lycii isoelectric focusing
-
 3.61
3.1.3.26 Peniophora lycii calculation from amino acid sequence
-
 4.35
3.1.3.26 Trametes pubescens calculation from amino acid sequence
-
 4.4
3.1.3.26 Ceriporia sp. calculation from amino acid sequence
-
 4.64
3.1.3.26 Agrocybe pediades calculation from amino acid sequence
-
 5
3.1.3.26 Ceriporia sp. calculation from amino acid sequence
-
 6.4