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Literature summary extracted from

  • Basten, D.E.J.W.; Dekker, P.J.T.; Schaap, P.J.
    Aminopeptidase C of Aspergillus niger is a novel phenylalanine aminopeptidase (2003), Appl. Environ. Microbiol., 1246-1250, 69.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.40
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.40 extracellular
-
 Aspergillus niger
-
-
3.4.22.40 intracellular
-
 Aspergillus niger 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.40 72000
-
 x * 72000, SDS-PAGE and deduced from gene sequence Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.40 Aspergillus niger Q8J219
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.40
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.40 189
-
 pH 5.2, 30°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.40 L-tyrosine-p-nitroanilide + H2O 72% of activity with phenylalanine-betanitroanilide Aspergillus niger L-tyrosine + 4-nitroaniline
-
 ?
3.4.22.40 phenylalanine-betanaphthylamide + H2O best substrate Aspergillus niger beta-naphthylamin + phenylalanine
-
 ?
3.4.22.40 phenylalanine-p-nitroanilide + H2O activity comparable to phenylalanine-betanitroanilide Aspergillus niger p-nitroaniline + phenylalanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.40 ? x * 72000, SDS-PAGE and deduced from gene sequence Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.4.22.40 ApsC
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.40 50
-
 1 h, pH 7.0, stable Aspergillus niger
3.4.22.40 60
-
 1 h, pH 7.0, 5% residual activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.40 5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.40 5 8 stable for 90 min Aspergillus niger