Literature summary extracted from

Beyer, D.; Kroll, H.P.; Endermann, R.; Schiffer, G.; Siegel, S.; Bauser, M.; Pohlmann, J.; Brands, M.; Ziegelbauer, K.; Haebich, D.; Eymann, C.; Brotz-Oesterhelt, H.
New class of bacterial phenylalanyl-tRNA synthetase inhibitors with high potency and broad-spectrum activity (2004), Antimicrob. Agents Chemother., 48, 525-532.

Inhibitors

EC Number	Inhibitors	Comment	Organism
6.1.1.20	chloramphenicol	-	Haemophilus influenzae
6.1.1.20	chloramphenicol	-	Moraxella catarrhalis
6.1.1.20	chloramphenicol	-	Staphylococcus aureus
6.1.1.20	chloramphenicol	-	Streptococcus pneumoniae
6.1.1.20	Methicillin	-	Haemophilus influenzae
6.1.1.20	Methicillin	-	Moraxella catarrhalis
6.1.1.20	Methicillin	-	Staphylococcus aureus
6.1.1.20	Methicillin	-	Streptococcus pneumoniae
6.1.1.20	muciproin	-	Escherichia coli
6.1.1.20	muciproin	-	Haemophilus influenzae
6.1.1.20	muciproin	-	Moraxella catarrhalis
6.1.1.20	muciproin	-	Staphylococcus aureus
6.1.1.20	muciproin	-	Streptococcus pneumoniae
6.1.1.20	phenyl-thiazolylurea-sulfonamides	strong inhibition, several derivatives, competitive to L-phenylalanine	Escherichia coli
6.1.1.20	phenyl-thiazolylurea-sulfonamides	strong inhibition, several derivatives, competitive to L-phenylalanine, cytotoxic effect	Haemophilus influenzae
6.1.1.20	phenyl-thiazolylurea-sulfonamides	strong inhibition, several derivatives, competitive to L-phenylalanine, cytotoxic effect	Moraxella catarrhalis
6.1.1.20	phenyl-thiazolylurea-sulfonamides	strong inhibition, several derivatives, competitive to L-phenylalanine, cytotoxic effect	Staphylococcus aureus
6.1.1.20	phenyl-thiazolylurea-sulfonamides	strong inhibition, several derivatives, competitive to L-phenylalanine, cytotoxic effect	Streptococcus pneumoniae
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethyl-chloroindol	-	Haemophilus influenzae
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethyl-chloroindol	-	Moraxella catarrhalis
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethyl-chloroindol	-	Staphylococcus aureus
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethyl-chloroindol	-	Streptococcus pneumoniae
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethyl-chloroindole	-	Escherichia coli
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethylindol	strong inhibition, competitive to L-phenylalanine, cytotoxic effects on CHO cells and bacteria	Haemophilus influenzae
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethylindol	strong inhibition, competitive to L-phenylalanine, cytotoxic effects on CHO cells and bacteria	Moraxella catarrhalis
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethylindol	strong inhibition, competitive to L-phenylalanine, cytotoxic effects on CHO cells and bacteria	Staphylococcus aureus
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethylindol	strong inhibition, competitive to L-phenylalanine, cytotoxic effects on CHO cells and bacteria	Streptococcus pneumoniae
6.1.1.20	phenyl-thiazolylurea-sulfonamido-aminoethylindole	strong inhibition, competitive to L-phenylalanine, cytotoxic effects on CHO cells and bacteria	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Staphylococcus aureus	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Haemophilus influenzae	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Escherichia coli	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Streptococcus pneumoniae	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Moraxella catarrhalis	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Staphylococcus aureus 133	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Streptococcus pneumoniae G9A	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Moraxella catarrhalis 489	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	Haemophilus influenzae Spain 7	-	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.20	Escherichia coli	-	purified enzyme, strain CP78 and mutant relA- strain CP79	-
6.1.1.20	Haemophilus influenzae	-	clinical isolate	-
6.1.1.20	Haemophilus influenzae Spain 7	-	clinical isolate	-
6.1.1.20	Moraxella catarrhalis	-	-	-
6.1.1.20	Moraxella catarrhalis 489	-	-	-
6.1.1.20	Staphylococcus aureus	-	-	-
6.1.1.20	Staphylococcus aureus 133	-	-	-
6.1.1.20	Streptococcus pneumoniae	-	-	-
6.1.1.20	Streptococcus pneumoniae G9A	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Staphylococcus aureus	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Haemophilus influenzae	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Escherichia coli	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Streptococcus pneumoniae	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Moraxella catarrhalis	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Staphylococcus aureus 133	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Streptococcus pneumoniae G9A	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Moraxella catarrhalis 489	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?
6.1.1.20	ATP + L-phenylalanine + tRNAPhe	-	Haemophilus influenzae Spain 7	AMP + diphosphate + L-phenylalanyl-tRNAPhe	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.1.1.20	CML33	-	Staphylococcus aureus
6.1.1.20	CML33	-	Haemophilus influenzae
6.1.1.20	CML33	-	Escherichia coli
6.1.1.20	CML33	-	Streptococcus pneumoniae
6.1.1.20	CML33	-	Moraxella catarrhalis
6.1.1.20	FRS	-	Staphylococcus aureus
6.1.1.20	FRS	-	Haemophilus influenzae
6.1.1.20	FRS	-	Escherichia coli
6.1.1.20	FRS	-	Streptococcus pneumoniae
6.1.1.20	FRS	-	Moraxella catarrhalis
6.1.1.20	HSPC173	-	Staphylococcus aureus
6.1.1.20	HSPC173	-	Haemophilus influenzae
6.1.1.20	HSPC173	-	Escherichia coli
6.1.1.20	HSPC173	-	Streptococcus pneumoniae
6.1.1.20	HSPC173	-	Moraxella catarrhalis
6.1.1.20	L-Phenylalanyl-tRNA synthetase	-	Staphylococcus aureus
6.1.1.20	L-Phenylalanyl-tRNA synthetase	-	Haemophilus influenzae
6.1.1.20	L-Phenylalanyl-tRNA synthetase	-	Escherichia coli
6.1.1.20	L-Phenylalanyl-tRNA synthetase	-	Streptococcus pneumoniae
6.1.1.20	L-Phenylalanyl-tRNA synthetase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanine translase	-	Staphylococcus aureus
6.1.1.20	Phenylalanine translase	-	Haemophilus influenzae
6.1.1.20	Phenylalanine translase	-	Escherichia coli
6.1.1.20	Phenylalanine translase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanine translase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanine--tRNA ligase	-	Staphylococcus aureus
6.1.1.20	Phenylalanine--tRNA ligase	-	Haemophilus influenzae
6.1.1.20	Phenylalanine--tRNA ligase	-	Escherichia coli
6.1.1.20	Phenylalanine--tRNA ligase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanine--tRNA ligase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanine-tRNA synthetase	-	Staphylococcus aureus
6.1.1.20	Phenylalanine-tRNA synthetase	-	Haemophilus influenzae
6.1.1.20	Phenylalanine-tRNA synthetase	-	Escherichia coli
6.1.1.20	Phenylalanine-tRNA synthetase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanine-tRNA synthetase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl transfer ribonucleic acid synthetase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl transfer ribonucleic acid synthetase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl transfer ribonucleic acid synthetase	-	Escherichia coli
6.1.1.20	Phenylalanyl transfer ribonucleic acid synthetase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl transfer ribonucleic acid synthetase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl-transfer ribonucleate synthetase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl-transfer ribonucleate synthetase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl-transfer ribonucleate synthetase	-	Escherichia coli
6.1.1.20	Phenylalanyl-transfer ribonucleate synthetase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl-transfer ribonucleate synthetase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl-transfer RNA ligase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl-transfer RNA ligase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl-transfer RNA ligase	-	Escherichia coli
6.1.1.20	Phenylalanyl-transfer RNA ligase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl-transfer RNA ligase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl-transfer RNA synthetase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl-transfer RNA synthetase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl-transfer RNA synthetase	-	Escherichia coli
6.1.1.20	Phenylalanyl-transfer RNA synthetase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl-transfer RNA synthetase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl-tRNA ligase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl-tRNA ligase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl-tRNA ligase	-	Escherichia coli
6.1.1.20	Phenylalanyl-tRNA ligase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl-tRNA ligase	-	Moraxella catarrhalis
6.1.1.20	Phenylalanyl-tRNA synthetase	-	Staphylococcus aureus
6.1.1.20	Phenylalanyl-tRNA synthetase	-	Haemophilus influenzae
6.1.1.20	Phenylalanyl-tRNA synthetase	-	Escherichia coli
6.1.1.20	Phenylalanyl-tRNA synthetase	-	Streptococcus pneumoniae
6.1.1.20	Phenylalanyl-tRNA synthetase	-	Moraxella catarrhalis
6.1.1.20	PheRS	-	Staphylococcus aureus
6.1.1.20	PheRS	-	Haemophilus influenzae
6.1.1.20	PheRS	-	Escherichia coli
6.1.1.20	PheRS	-	Streptococcus pneumoniae
6.1.1.20	PheRS	-	Moraxella catarrhalis
6.1.1.20	Synthetase, phenylalanyl-transfer ribonucleate	-	Staphylococcus aureus
6.1.1.20	Synthetase, phenylalanyl-transfer ribonucleate	-	Haemophilus influenzae
6.1.1.20	Synthetase, phenylalanyl-transfer ribonucleate	-	Escherichia coli
6.1.1.20	Synthetase, phenylalanyl-transfer ribonucleate	-	Streptococcus pneumoniae
6.1.1.20	Synthetase, phenylalanyl-transfer ribonucleate	-	Moraxella catarrhalis

Cofactor

EC Number	Cofactor	Comment	Organism
6.1.1.20	ATP	-	Staphylococcus aureus
6.1.1.20	ATP	-	Haemophilus influenzae
6.1.1.20	ATP	-	Escherichia coli
6.1.1.20	ATP	-	Streptococcus pneumoniae
6.1.1.20	ATP	-	Moraxella catarrhalis

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
6.1.1.20	additional information	-	additional information	-	Staphylococcus aureus
6.1.1.20	additional information	-	additional information	-	Haemophilus influenzae
6.1.1.20	additional information	-	additional information	-	Escherichia coli
6.1.1.20	additional information	-	additional information	-	Streptococcus pneumoniae
6.1.1.20	additional information	-	additional information	-	Moraxella catarrhalis
6.1.1.20	0.000021	-	phenyl-thiazolylurea-sulfonamido-aminoethylindole	pH 7.6, 22°C	Escherichia coli