Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takahashi, T.; Ikegami-Kawai, M.; Okuda, R.; Suzuki, K.
    A fluorometric Morgan-Elson method for hyaluronidase activity (2003), Anal. Biochem., 322, 257-263.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.35 additional information
-
 additional information kinetics Homo sapiens
3.2.1.35 additional information
-
 additional information kinetics Bos taurus
3.2.1.35 additional information
-
 additional information kinetics Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 NaCl
-
 Homo sapiens
3.2.1.35 NaCl
-
 Bos taurus
3.2.1.35 NaCl
-
 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus
-
-
-
3.2.1.35 Homo sapiens
-
-
-
3.2.1.35 Oryctolagus cuniculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Homo sapiens
a
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Bos taurus
a
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 commercial preparation
-
 Bos taurus
-
3.2.1.35 serum
-
 Homo sapiens
-
3.2.1.35 serum
-
 Oryctolagus cuniculus
-
3.2.1.35 testicle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.35 additional information
-
 activity in serum samples, development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Homo sapiens
3.2.1.35 additional information
-
 activity in serum samples, development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Oryctolagus cuniculus
3.2.1.35 additional information
-
 development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Oryctolagus cuniculus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 HAase
-
 Bos taurus
3.2.1.35 hyaluronidase
-
 Homo sapiens
3.2.1.35 hyaluronidase
-
 Bos taurus
3.2.1.35 hyaluronidase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Homo sapiens
3.2.1.35 37
-
 assay at Bos taurus
3.2.1.35 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 3.9
-
 assay at Oryctolagus cuniculus
3.2.1.35 3.9
-
 assay at Homo sapiens
3.2.1.35 6
-
 assay at Bos taurus