EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.1 | D-Aspartate | - |
Escherichia coli | |
4.3.1.1 | o-phospho-D-serine | - |
Escherichia coli |
EC Number | Application | Comment | Organism |
---|---|---|---|
4.3.1.1 | biotechnology | overview on commercial applications | Escherichia coli |
4.3.1.1 | medicine | involvement of enzyme in blood clotting and activation of plasminogen, overview | Haemophilus influenzae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.1.1 | overview | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.1.1 | C141S/C274A | elimination of sensitivity to inactivation | Escherichia coli |
4.3.1.1 | K140I | Km value 10fold higher than wild type, comparable increase in Ki for competitive inhibitors | Escherichia coli |
4.3.1.1 | additional information | overview | Escherichia coli |
4.3.1.1 | additional information | overview | Hafnia alvei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.1 | 2,3-diphosphoglycerate | - |
Escherichia coli | |
4.3.1.1 | 3-nitropropanoate | - |
Escherichia coli | |
4.3.1.1 | D-malate | - |
Escherichia coli | |
4.3.1.1 | DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
4.3.1.1 | additional information | overview on inhibitors | Escherichia coli | |
4.3.1.1 | o-phospho-D-serine | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.1 | Mg2+ | activation | Hafnia alvei | |
4.3.1.1 | Mn2+ | activation | Hafnia alvei | |
4.3.1.1 | additional information | absolute requirement for divalent metal ion at higher pH | Escherichia coli | |
4.3.1.1 | additional information | no activation by Ca2+, minimal activation by Co2+, pH-dependent activation by divalent cations at high pH values | Hafnia alvei | |
4.3.1.1 | Zn2+ | activation | Hafnia alvei | |
4.3.1.1 | Zn2+ | most potent activating metal ion | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.1.1 | 52000 | - |
4 * 52000, SDS-PAGE | Escherichia coli |
4.3.1.1 | 52000 | - |
4 * 52000, overview | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.1 | Escherichia coli | - |
- |
- |
4.3.1.1 | Haemophilus influenzae | - |
- |
- |
4.3.1.1 | Hafnia alvei | - |
- |
- |
4.3.1.1 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.1 | overview | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.3.1.1 | L-aspartate = fumarate + NH3 | mechanism | Escherichia coli | |
4.3.1.1 | L-aspartate = fumarate + NH3 | mechanism: overview | Hafnia alvei |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.3.1.1 | denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Escherichia coli |
4.3.1.1 | denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.1 | L-aspartate | - |
Escherichia coli | fumarate + NH3 | - |
r | |
4.3.1.1 | additional information | no activity with D-aspartic acid or crotonic acid, overview substrate specificity | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.1 | homotetramer | 4 * 52000, overview | Pseudomonas fluorescens |
4.3.1.1 | More | overview | Hafnia alvei |
4.3.1.1 | tetramer | 4 * 52000, SDS-PAGE | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.1 | 0.2 | - |
o-phospho-D-serine | - |
Escherichia coli | |
4.3.1.1 | 0.66 | - |
D-malate | - |
Escherichia coli | |
4.3.1.1 | 0.66 | - |
DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
4.3.1.1 | 0.83 | - |
3-nitropropanoate | - |
Escherichia coli | |
4.3.1.1 | 1.1 | - |
2,3-diphosphoglycerate | - |
Escherichia coli |